Separation and identification of the uremic middle molecules

Mei Yang, Jian Da Zhang, Peng Wang, Xiao Hang Liu, Zhi Yuan, Hong Fu Liu, Hong Tao Yang, Shu Fen Yang, Jing Li Zhao

Research output: Contribution to journalArticlepeer-review


Sera and urine from patients with severe uremia and healthy persons were separated by means of gel permeation chromatography on Sephadex G-15 column with N(C2H5)3-H2CO3 buffer as the eluent. Two middle molecular peaks(A and B) were detected at 206 nm in normal urine, uremic serum and uremic urine, but these two peaks were hardly observed in the profile of normal sera. In contrast, the absorption at 206 nm of fractions A and B from uremic urine were smaller than that of fractions A and B from normal urine. Fractions A from normal urine, uremic serum and urine were collected and resolved into 3 subpeaks at 254 nm by high performance liquid chromatography. Two of these subpeaks, A- I and A- I, were detected in uremic serum, normal urine and uremic urine. The results of MALDI-TOF-MS revealed that the fraction A- I from both uremic serum and normal urine contained a component with molecular weight 1214, which could hardly be seen in the fraction A- I of uremic urine.

Original languageEnglish
Pages (from-to)677-679
Number of pages3
JournalKao Teng Hsueh Hsiao Hua Heush Hsueh Pao/ Chemical Journal of Chinese Universities
Issue number4
StatePublished - Apr 2005
Externally publishedYes


  • High performance liquid chromatography
  • Middle molecule
  • Time-of-flight mass spectrometry
  • Uremia


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