Semaphorins guide the entry of dendritic cells into the lymphatics by activating myosin II

Hyota Takamatsu; Noriko Takegahara; Yukinobu Nakagawa; Michio Tomura; Masahiko Taniguchi; Roland H. Friedel; Helen Rayburn; Marc Tessier-Lavigne; Yutaka Yoshida; Tatsusada Okuno; Masayuki Mizui; Sujin Kang; Satoshi Nojima; Tohru Tsujimura; Yuji Nakatsuji; Ichiro Katayama; Toshihiko Toyofuku; Hitoshi Kikutani; Atsushi Kumanogoh

doi:10.1038/ni.1885

Semaphorins guide the entry of dendritic cells into the lymphatics by activating myosin II

Hyota Takamatsu, Noriko Takegahara, Yukinobu Nakagawa, Michio Tomura, Masahiko Taniguchi, Roland H. Friedel, Helen Rayburn, Marc Tessier-Lavigne, Yutaka Yoshida, Tatsusada Okuno, Masayuki Mizui, Sujin Kang, Satoshi Nojima, Tohru Tsujimura, Yuji Nakatsuji, Ichiro Katayama, Toshihiko Toyofuku, Hitoshi Kikutani, Atsushi Kumanogoh

Research output: Contribution to journal › Article › peer-review

182 Scopus citations

Abstract

The recirculation of leukocytes is essential for proper immune responses. However, the molecular mechanisms thaT_regulate the entry of leukocytes into the lymphatics remain unclear. Here we show that plexin-A1, a principal receptor component for class III and class VI semaphorins, was crucially involved in the entry of dendritic cells (DCs) into the lymphatics. Additionally, we show that the semaphorin Sema3A, but not Sema6C or Sema6D, was required for DC transmigration and that Sema3A produced by the lymphatics promoted actomyosin contraction at the trailing edge of migrating DCs. Our findings not only demonstrate that semaphorin signals are involved in DC trafficking but also identify a previously unknown mechanism that induces actomyosin contraction as these cells pass through narrow gaps.

Original language	English
Pages (from-to)	594-600
Number of pages	7
Journal	Nature Immunology
Volume	11
Issue number	7
DOIs	https://doi.org/10.1038/ni.1885
State	Published - Jul 2010
Externally published	Yes

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Takamatsu, H., Takegahara, N., Nakagawa, Y., Tomura, M., Taniguchi, M., Friedel, R. H., Rayburn, H., Tessier-Lavigne, M., Yoshida, Y., Okuno, T., Mizui, M., Kang, S., Nojima, S., Tsujimura, T., Nakatsuji, Y., Katayama, I., Toyofuku, T., Kikutani, H., & Kumanogoh, A. (2010). Semaphorins guide the entry of dendritic cells into the lymphatics by activating myosin II. Nature Immunology, 11(7), 594-600. https://doi.org/10.1038/ni.1885

@article{29401523d97f4ef487486c458f650467,

title = "Semaphorins guide the entry of dendritic cells into the lymphatics by activating myosin II",

abstract = "The recirculation of leukocytes is essential for proper immune responses. However, the molecular mechanisms thaTregulate the entry of leukocytes into the lymphatics remain unclear. Here we show that plexin-A1, a principal receptor component for class III and class VI semaphorins, was crucially involved in the entry of dendritic cells (DCs) into the lymphatics. Additionally, we show that the semaphorin Sema3A, but not Sema6C or Sema6D, was required for DC transmigration and that Sema3A produced by the lymphatics promoted actomyosin contraction at the trailing edge of migrating DCs. Our findings not only demonstrate that semaphorin signals are involved in DC trafficking but also identify a previously unknown mechanism that induces actomyosin contraction as these cells pass through narrow gaps.",

author = "Hyota Takamatsu and Noriko Takegahara and Yukinobu Nakagawa and Michio Tomura and Masahiko Taniguchi and Friedel, \{Roland H.\} and Helen Rayburn and Marc Tessier-Lavigne and Yutaka Yoshida and Tatsusada Okuno and Masayuki Mizui and Sujin Kang and Satoshi Nojima and Tohru Tsujimura and Yuji Nakatsuji and Ichiro Katayama and Toshihiko Toyofuku and Hitoshi Kikutani and Atsushi Kumanogoh",

note = "Funding Information: We thank D.D. Ginty and A.L. Kolodkin (Howard Hughes Medical Institute) for neuropilin-1-mutant mice; W.R. Heath (University of Melbourne) for OT-II-transgenic mice; and T. Yazawa for technical support. Supported by the Japan Society for the Promotion of Science (Research Fellowships for Young Scientists to H.T.), the US National Institutes of Health (R01NS065048 to Y.Y.), the Ministry of Education, Culture, Sports, Science and Technology of Japan, the Ministry of Health, Labour and Welfare, the Program for Promotion of Fundamental Studies in Health Sciences of the National Institute of Biomedical Innovation (A.K.), the Target Protein Research Program of the Japan Science and Technology Agency (T.T. and A.K.), the Uehara Memorial Foundation (A.K.) and the Takeda Scientific Foundation (T.T., N.T. and A.K.).",

year = "2010",

month = jul,

doi = "10.1038/ni.1885",

language = "English",

volume = "11",

pages = "594--600",

journal = "Nature Immunology",

issn = "1529-2908",

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Takamatsu, H, Takegahara, N, Nakagawa, Y, Tomura, M, Taniguchi, M, Friedel, RH, Rayburn, H, Tessier-Lavigne, M, Yoshida, Y, Okuno, T, Mizui, M, Kang, S, Nojima, S, Tsujimura, T, Nakatsuji, Y, Katayama, I, Toyofuku, T, Kikutani, H & Kumanogoh, A 2010, 'Semaphorins guide the entry of dendritic cells into the lymphatics by activating myosin II', Nature Immunology, vol. 11, no. 7, pp. 594-600. https://doi.org/10.1038/ni.1885

TY - JOUR

T1 - Semaphorins guide the entry of dendritic cells into the lymphatics by activating myosin II

AU - Takamatsu, Hyota

AU - Takegahara, Noriko

AU - Nakagawa, Yukinobu

AU - Tomura, Michio

AU - Taniguchi, Masahiko

AU - Friedel, Roland H.

AU - Rayburn, Helen

AU - Tessier-Lavigne, Marc

AU - Yoshida, Yutaka

AU - Okuno, Tatsusada

AU - Mizui, Masayuki

AU - Kang, Sujin

AU - Nojima, Satoshi

AU - Tsujimura, Tohru

AU - Nakatsuji, Yuji

AU - Katayama, Ichiro

AU - Toyofuku, Toshihiko

AU - Kikutani, Hitoshi

AU - Kumanogoh, Atsushi

N1 - Funding Information: We thank D.D. Ginty and A.L. Kolodkin (Howard Hughes Medical Institute) for neuropilin-1-mutant mice; W.R. Heath (University of Melbourne) for OT-II-transgenic mice; and T. Yazawa for technical support. Supported by the Japan Society for the Promotion of Science (Research Fellowships for Young Scientists to H.T.), the US National Institutes of Health (R01NS065048 to Y.Y.), the Ministry of Education, Culture, Sports, Science and Technology of Japan, the Ministry of Health, Labour and Welfare, the Program for Promotion of Fundamental Studies in Health Sciences of the National Institute of Biomedical Innovation (A.K.), the Target Protein Research Program of the Japan Science and Technology Agency (T.T. and A.K.), the Uehara Memorial Foundation (A.K.) and the Takeda Scientific Foundation (T.T., N.T. and A.K.).

PY - 2010/7

Y1 - 2010/7

N2 - The recirculation of leukocytes is essential for proper immune responses. However, the molecular mechanisms thaTregulate the entry of leukocytes into the lymphatics remain unclear. Here we show that plexin-A1, a principal receptor component for class III and class VI semaphorins, was crucially involved in the entry of dendritic cells (DCs) into the lymphatics. Additionally, we show that the semaphorin Sema3A, but not Sema6C or Sema6D, was required for DC transmigration and that Sema3A produced by the lymphatics promoted actomyosin contraction at the trailing edge of migrating DCs. Our findings not only demonstrate that semaphorin signals are involved in DC trafficking but also identify a previously unknown mechanism that induces actomyosin contraction as these cells pass through narrow gaps.

AB - The recirculation of leukocytes is essential for proper immune responses. However, the molecular mechanisms thaTregulate the entry of leukocytes into the lymphatics remain unclear. Here we show that plexin-A1, a principal receptor component for class III and class VI semaphorins, was crucially involved in the entry of dendritic cells (DCs) into the lymphatics. Additionally, we show that the semaphorin Sema3A, but not Sema6C or Sema6D, was required for DC transmigration and that Sema3A produced by the lymphatics promoted actomyosin contraction at the trailing edge of migrating DCs. Our findings not only demonstrate that semaphorin signals are involved in DC trafficking but also identify a previously unknown mechanism that induces actomyosin contraction as these cells pass through narrow gaps.

UR - https://www.scopus.com/pages/publications/77953808714

U2 - 10.1038/ni.1885

DO - 10.1038/ni.1885

M3 - Article

C2 - 20512151

AN - SCOPUS:77953808714

SN - 1529-2908

VL - 11

SP - 594

EP - 600

JO - Nature Immunology

JF - Nature Immunology

IS - 7

ER -

Semaphorins guide the entry of dendritic cells into the lymphatics by activating myosin II

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