Self-Assembled Lanthanide Antenna Glutathione Sensor for the Study of Immune Cells

Francisco Fueyo-González, Laura Espinar-Barranco, Rosario Herranz, Ibon Alkorta, Luis Crovetto, Miguel Fribourg, Jose Manuel Paredes, Angel Orte, Juan A. González-Vera

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

The small molecule 8-methoxy-2-oxo-1,2,4,5-tetrahydrocyclopenta[de]quinoline-3-carboxylic acid (2b) behaves as a reactive non-fluorescent Michael acceptor, which after reaction with thiols becomes fluorescent, and an efficient Eu3+ antenna, after self-assembling with this cation in water. This behavior makes 2b a highly selective GSH biosensor, which has demonstrated high potential for studies in murine and human cells of the immune system (CD4+ T, CD8+ T, and B cells) using flow cytometry. GSH can be monitored by the fluorescence of the product of addition to 2b (445 nm) or by the luminescence of Eu3+ (592 nm). 2b was able to capture baseline differences in GSH intracellular levels among murine and human CD4+ T, CD8+ T, and B cells. We also successfully used 2b to monitor intracellular changes in GSH associated with the metabolic variations governing the induction of CD4+ naïve T cells into regulatory T cells (TREG).

Original languageEnglish
Pages (from-to)322-330
Number of pages9
JournalACS Sensors
Volume7
Issue number1
DOIs
StatePublished - 28 Jan 2022

Keywords

  • T
  • T cells
  • flow cytometry
  • glutathione
  • lanthanide
  • luminescent sensor
  • self-assembled antenna
  • time-resolved luminescence

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