TY - JOUR
T1 - Selective RNA editing and subunit assembly of native glutamate receptors
AU - Puchalski, Ralph B.
AU - Louis, Jean Claude
AU - Brose, Nils
AU - Traynelis, Stephen F.
AU - Egebjerg, Jan
AU - Kukekov, Valery
AU - J.Wenthold, Robert
AU - Rogers, Scott W.
AU - Lin, Fan
AU - Moran, Thomas
AU - Morrison, John H.
AU - Heinemann, Stephen F.
N1 - Funding Information:
We would like to thank our colleagues for help, materials, and advice, especially Jim Boulter, Lora Beasley, Michael Hollmann, Jane Sullivan, and Yael Stern-Bach. We are grateful to Roger Papke for designing and constructing the rapid perfusion system. We thank Craig Blackstone and Richard Huganir (Johns Hopkins University) for their generous gifts of antibodies, Peter Seeburg (Heidelberg University, Heidelberg, Federal Republic of Germany) for the use of the CluR-D cDNA clone to characterize the specificity of antibodies, and Andrew Peterson (University of California at San Francisco) for the KA2 clone. R. J. W. thanks Peter Seeburg for the KAI and KA2 clones and Shigetada Nakanishi (Kyoto University, Kyoto, Japan) for the NMDARl clone. We thank Doris Patneau and Vittorio Gallo (NIH) for sharing unpublished results of their characterization of oligodendrocyte progenitor cells. We thank the people in the Salk photography and graphics department, especially Liz Grabowski, for the construction and duplication of the figures. This work was supported in part by postdoctoral fellowships from the Epilepsy Foundation of America (R. 6. P. and S. F. T.), the NIH (R. B. P., NS09029-02; S. F. T., NS08549-03), the German Research Foundation (N. B.), the Danish Medical Research Council fJ. E.), and the Howard Hughes Medical Institute (F. L.), by grants from the NIH fJ. H. M., AC06647; S. W. R., NSIR293099082), and by the McKnight Endowment Fund for Neuroscience and the National Institute for Neurological Disorders and Stroke (S. F. H., ROlNS28709).
PY - 1994/7
Y1 - 1994/7
N2 - RNA editing and subunit assembly of ionotropic glutamate receptors (GIuRs) were examined in an oligodendrocyte progenitor cell line, CG4, which expresses GluR2-GluR4, GluR6, GluR7, KA1, and KA2. AMPA-evoked currents rapidly desensitize, whereas kainate-evoked currents contain a steady-state component with a nearly linear current-voltage relation and a fast desensitizing component that is inwardly rectifying. The Q/R site is edited >95% to the arginine codon in GluR2(Q607) mRNA, and <5% in GluR6(Q621) mRNA. Immunoprecipitation experiments demonstrate that GluR6 and/or GluR7 subunits assemble with KA2, but not with GluR2-GluR4. These results indicate that oligodendrocyte progenitor cells selectively edit and assemble glutamate receptors into at least two functionally and structurally distinct heteromeric channels.
AB - RNA editing and subunit assembly of ionotropic glutamate receptors (GIuRs) were examined in an oligodendrocyte progenitor cell line, CG4, which expresses GluR2-GluR4, GluR6, GluR7, KA1, and KA2. AMPA-evoked currents rapidly desensitize, whereas kainate-evoked currents contain a steady-state component with a nearly linear current-voltage relation and a fast desensitizing component that is inwardly rectifying. The Q/R site is edited >95% to the arginine codon in GluR2(Q607) mRNA, and <5% in GluR6(Q621) mRNA. Immunoprecipitation experiments demonstrate that GluR6 and/or GluR7 subunits assemble with KA2, but not with GluR2-GluR4. These results indicate that oligodendrocyte progenitor cells selectively edit and assemble glutamate receptors into at least two functionally and structurally distinct heteromeric channels.
UR - https://www.scopus.com/pages/publications/0027941193
U2 - 10.1016/0896-6273(94)90464-2
DO - 10.1016/0896-6273(94)90464-2
M3 - Article
C2 - 7519023
AN - SCOPUS:0027941193
SN - 0896-6273
VL - 13
SP - 131
EP - 147
JO - Neuron
JF - Neuron
IS - 1
ER -