@inbook{f802d7d0239d46d9abf757e7eef72dfe,
title = "Selective Anti-AAV Antibody Depletion by Hemapheresis and Immunoadsorption",
abstract = "One of the greatest barriers for the use of adeno-associated vectors (AAV) in gene therapy is the presence of pre-existing antibodies against AAV in the general population. Since many of the anti-AAV antibodies have the ability to neutralize the transduction target tissues, even patients with low antibody titers must be excluded from clinical trials or therapy. In recent years, various methods have been proposed to overcome this problem, unfortunately with limited success. In this chapter, we describe in detail a protocol for hemapheresis with an immunoadsorption matrix to remove specifically anti-AAV antibodies in an in vivo rat model. Furthermore, this chapter describes in detail the methods to determine the efficiency of hemapheresis and immunoadsorption.",
keywords = "Adeno-associated virus, Hemapheresis, Immunoadsorption, Neutralization assay, Neutralizing antibodies, Plasmapheresis",
author = "Alejandro Orlowski and Thomas Weber",
note = "Funding Information: This work was supported by NHLBI grant HL131404 (T.W.) and a Transatlantic Network of Excellence grant (14 CVD 03) by the Leducq Foundation (T.W.). Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2707-5_18",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "235--248",
booktitle = "Methods in Molecular Biology",
}