TY - JOUR
T1 - Selective antagonism of calcitonin-induced osteoclastic quiescence (Q effect) by human calcitonin gene-related peptide-(Val8Phe37)
AU - Towhidul Alam, A. S.M.
AU - Moonga, Baljit S.
AU - Bevis, Peter J.R.
AU - Huang, Christopher L.H.
AU - Zaidi, Mone
N1 - Funding Information:
Acknowledements: The studies were supported in part by the Medical Research Council, UK (MZ), Arthritis and Rheumatism Council, UK (MZ) and Leverhulme Trust, UK (MZ). ASMTA is a recipient of the Overseas Research Students Award. The authors are grateful to Professor Iain MacIntyre for constructive advice on the manuscript.
PY - 1991/8/30
Y1 - 1991/8/30
N2 - Exposure of isolated rat osteoclasts to calcitonin (CT) leads to an abrupt cessation of cell motility (Q effect) followed by cell retraction (R effect). We have previously shown that these effects are mediated by two G proteins that appear to activate separate post-receptor pathways. The present study demonstrates that the Q but not the R effect of CT (0.006 μM) is abolished in the presence of human calcitonin gene-related peptide (CGRP)-(Val8Phe37) (0.5 μM), a fragment analogue of human CGRP. This selective antagonism suggests that the Q effect could result from an action of CT upon a site that is distinct from that producing the R effect. The former site ('amylin site') also appears to interact with related peptides, amylin and CGRP, whilst the latter site ('CT site') specifically interacts with CT.
AB - Exposure of isolated rat osteoclasts to calcitonin (CT) leads to an abrupt cessation of cell motility (Q effect) followed by cell retraction (R effect). We have previously shown that these effects are mediated by two G proteins that appear to activate separate post-receptor pathways. The present study demonstrates that the Q but not the R effect of CT (0.006 μM) is abolished in the presence of human calcitonin gene-related peptide (CGRP)-(Val8Phe37) (0.5 μM), a fragment analogue of human CGRP. This selective antagonism suggests that the Q effect could result from an action of CT upon a site that is distinct from that producing the R effect. The former site ('amylin site') also appears to interact with related peptides, amylin and CGRP, whilst the latter site ('CT site') specifically interacts with CT.
UR - http://www.scopus.com/inward/record.url?scp=0026079426&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(91)91345-D
DO - 10.1016/0006-291X(91)91345-D
M3 - Article
C2 - 1883346
AN - SCOPUS:0026079426
SN - 0006-291X
VL - 179
SP - 134
EP - 139
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -