Segregation of a missense mutation in the amyloid precursor protein gene with familial Alzheimer's disease

Alison Goate, Marie Christine Chartier-Harlin, Mike Mullan, Jeremy Brown, Fiona Crawford, Liana Fidani, Luis Giuffra, Andrew Haynes, Nick Irving, Louise James, Rebecca Mant, Phillippa Newton, Karen Rooke, Penelope Roques, Chris Talbot, Margaret Pericak-Vance, Alien Roses, Robert Williamson, Martin Rossor, Mike OwenJohn Hardy

Research output: Contribution to journalArticlepeer-review

3782 Scopus citations


A LOCUS segregating with familial Alzheimer's disease (AD) has been mapped to chromosome 21 (ref. 1), close to the amyloid precursor protein (APP) gene2-5. Recombinants between the APP gene and the AD locus have been reported6-8 which seemed to exclude it as the site of the mutation causing familial AD. But recent genetic analysis of a large number of AD families has demonstrated that the disease is heterogeneous9. Families with late-onset AD do not show linkage to chromosome 21 markers9,10. Some families with early-onset AD show linkage to chromosome 21 markers, but some do not8,9,11. This has led to the suggestion that there is non-allelic genetic heterogeneity even within early onset familial AD8,9. To avoid the problems that heterogeneity poses for genetic analysis, we have examined the cosegregation of AD and markers along the long arm of chromosome 21 in a single family with AD confirmed by autopsy. Here we demonstrate that in this kindred, which shows linkage to chromosome 21 markers, there is a point mutation in the APP gene. This mutation causes an amino-acid substitution (Val→Ile) close to the carboxy terminus of the β-amyloid peptide. Screening other cases of familial AD revealed a second unrelated family in which this variant occurs. This suggests that some cases of AD could be caused by mutations in the APP gene.

Original languageEnglish
Pages (from-to)704-706
Number of pages3
Issue number6311
StatePublished - 1991
Externally publishedYes


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