Segments of chromosomal DNA from Rhynchosciara americana that undergo additional rounds of DNA replication in the salivary gland DNA puffs have only weak ARS activity in yeast

Sarah Millar; David C. Hayward; Christopher A. Read; Michael J. Browne; Roberto V. Santelli; Felipe Garcia Vallejo; Manuel T. Pueyo; Arnaldo Zaha; David M. Glover; Francisco J.S. Lara

doi:10.1016/0378-1119(85)90297-5

Segments of chromosomal DNA from Rhynchosciara americana that undergo additional rounds of DNA replication in the salivary gland DNA puffs have only weak ARS activity in yeast

Sarah Millar
, David C. Hayward
, Christopher A. Read
, Michael J. Browne
, Roberto V. Santelli
, Felipe Garcia Vallejo
, Manuel T. Pueyo
, Arnaldo Zaha
, David M. Glover
, Francisco J.S. Lara

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

We have constructed a library of recombinant phage containing DNA from salivary gland chromosomes of Rhynchosciara americana. We have isolated phage from this library that carry sequences homologous to cDNA clones that hybridise in situ to the DNA puffs at the polytene chromosome regions C3 and C8. This has enabled us to demonstrate a 16-fold amplification of the genomic DNA sequences at these regions during DNA-puffing. At the C8 site there is a sequence element that has characteristics of 'scrambled' moderately repetitive DNA. This is located within 3 kb from the gene encoding a 1.95-kb mRNA. We have assayed restriction fragments from the two DNA puffs for Ars activity in yeast. The only strong Ars activity is associated with a part of the moderately repetitive DNA element from the C8 puff which is not present at this site in all animals.

Original language	English
Pages (from-to)	81-86
Number of pages	6
Journal	Gene
Volume	34
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(85)90297-5
State	Published - 1985
Externally published	Yes

Keywords

Recombinant DNA
gene amplification
genomic clones
yeast transformation
λ phage vector

Access to Document

10.1016/0378-1119(85)90297-5

Cite this

Millar, S., Hayward, D. C., Read, C. A., Browne, M. J., Santelli, R. V., Vallejo, F. G., Pueyo, M. T., Zaha, A., Glover, D. M., & Lara, F. J. S. (1985). Segments of chromosomal DNA from Rhynchosciara americana that undergo additional rounds of DNA replication in the salivary gland DNA puffs have only weak ARS activity in yeast. Gene, 34(1), 81-86. https://doi.org/10.1016/0378-1119(85)90297-5

@article{df5a8291417441b1a339fefe781946b1,

title = "Segments of chromosomal DNA from Rhynchosciara americana that undergo additional rounds of DNA replication in the salivary gland DNA puffs have only weak ARS activity in yeast",

abstract = "We have constructed a library of recombinant phage containing DNA from salivary gland chromosomes of Rhynchosciara americana. We have isolated phage from this library that carry sequences homologous to cDNA clones that hybridise in situ to the DNA puffs at the polytene chromosome regions C3 and C8. This has enabled us to demonstrate a 16-fold amplification of the genomic DNA sequences at these regions during DNA-puffing. At the C8 site there is a sequence element that has characteristics of 'scrambled' moderately repetitive DNA. This is located within 3 kb from the gene encoding a 1.95-kb mRNA. We have assayed restriction fragments from the two DNA puffs for Ars activity in yeast. The only strong Ars activity is associated with a part of the moderately repetitive DNA element from the C8 puff which is not present at this site in all animals.",

keywords = "Recombinant DNA, gene amplification, genomic clones, yeast transformation, λ phage vector",

author = "Sarah Millar and Hayward, \{David C.\} and Read, \{Christopher A.\} and Browne, \{Michael J.\} and Santelli, \{Roberto V.\} and Vallejo, \{Felipe Garcia\} and Pueyo, \{Manuel T.\} and Arnaldo Zaha and Glover, \{David M.\} and Lara, \{Francisco J.S.\}",

note = "Funding Information: This work was supportedb y grants from the StiftungV olkswagenwerkth, eF undacaod eAmparo a Pesquisad o Estado de S?LoP aulo, Financiadora National de Projetos (FINEP) and the Conselho National de DesenvolvimentoC ientifico e Tech-nologico( CNPq) to F.J.S.L. and from the Cancer Research Campaign( CRC) to D.M.G. D.M.G. holds a CareerD evelopmenAt ward from the CRC and also receiveda travel fellowshipt o visit the Universidaded e SBo Paul0 under the exchange programo f theR oyal Societya ndt heC NPq. R.V. S. receiveda fellowshipf rom CNPq for a sabbatical visitt o theI mperialC ollege.F .G.V. receiveds upport from the PNUD Schemeo f the United Nations. M.J.B. was a visitingw orkerf rom BeechamsP har-maceuticals. S.M. holds a Medical Research CouncilR esearchS tudentshipa ndD.C.H. received an undergraduates upport grant from Wiltshire County Council.",

year = "1985",

doi = "10.1016/0378-1119(85)90297-5",

language = "English",

volume = "34",

pages = "81--86",

journal = "Gene",

issn = "0378-1119",

publisher = "Elsevier B.V.",

number = "1",

}

Millar, S, Hayward, DC, Read, CA, Browne, MJ, Santelli, RV, Vallejo, FG, Pueyo, MT, Zaha, A, Glover, DM & Lara, FJS 1985, 'Segments of chromosomal DNA from Rhynchosciara americana that undergo additional rounds of DNA replication in the salivary gland DNA puffs have only weak ARS activity in yeast', Gene, vol. 34, no. 1, pp. 81-86. https://doi.org/10.1016/0378-1119(85)90297-5

TY - JOUR

T1 - Segments of chromosomal DNA from Rhynchosciara americana that undergo additional rounds of DNA replication in the salivary gland DNA puffs have only weak ARS activity in yeast

AU - Millar, Sarah

AU - Hayward, David C.

AU - Read, Christopher A.

AU - Browne, Michael J.

AU - Santelli, Roberto V.

AU - Vallejo, Felipe Garcia

AU - Pueyo, Manuel T.

AU - Zaha, Arnaldo

AU - Glover, David M.

AU - Lara, Francisco J.S.

N1 - Funding Information: This work was supportedb y grants from the StiftungV olkswagenwerkth, eF undacaod eAmparo a Pesquisad o Estado de S?LoP aulo, Financiadora National de Projetos (FINEP) and the Conselho National de DesenvolvimentoC ientifico e Tech-nologico( CNPq) to F.J.S.L. and from the Cancer Research Campaign( CRC) to D.M.G. D.M.G. holds a CareerD evelopmenAt ward from the CRC and also receiveda travel fellowshipt o visit the Universidaded e SBo Paul0 under the exchange programo f theR oyal Societya ndt heC NPq. R.V. S. receiveda fellowshipf rom CNPq for a sabbatical visitt o theI mperialC ollege.F .G.V. receiveds upport from the PNUD Schemeo f the United Nations. M.J.B. was a visitingw orkerf rom BeechamsP har-maceuticals. S.M. holds a Medical Research CouncilR esearchS tudentshipa ndD.C.H. received an undergraduates upport grant from Wiltshire County Council.

PY - 1985

Y1 - 1985

N2 - We have constructed a library of recombinant phage containing DNA from salivary gland chromosomes of Rhynchosciara americana. We have isolated phage from this library that carry sequences homologous to cDNA clones that hybridise in situ to the DNA puffs at the polytene chromosome regions C3 and C8. This has enabled us to demonstrate a 16-fold amplification of the genomic DNA sequences at these regions during DNA-puffing. At the C8 site there is a sequence element that has characteristics of 'scrambled' moderately repetitive DNA. This is located within 3 kb from the gene encoding a 1.95-kb mRNA. We have assayed restriction fragments from the two DNA puffs for Ars activity in yeast. The only strong Ars activity is associated with a part of the moderately repetitive DNA element from the C8 puff which is not present at this site in all animals.

AB - We have constructed a library of recombinant phage containing DNA from salivary gland chromosomes of Rhynchosciara americana. We have isolated phage from this library that carry sequences homologous to cDNA clones that hybridise in situ to the DNA puffs at the polytene chromosome regions C3 and C8. This has enabled us to demonstrate a 16-fold amplification of the genomic DNA sequences at these regions during DNA-puffing. At the C8 site there is a sequence element that has characteristics of 'scrambled' moderately repetitive DNA. This is located within 3 kb from the gene encoding a 1.95-kb mRNA. We have assayed restriction fragments from the two DNA puffs for Ars activity in yeast. The only strong Ars activity is associated with a part of the moderately repetitive DNA element from the C8 puff which is not present at this site in all animals.

KW - Recombinant DNA

KW - gene amplification

KW - genomic clones

KW - yeast transformation

KW - λ phage vector

UR - https://www.scopus.com/pages/publications/0021824586

U2 - 10.1016/0378-1119(85)90297-5

DO - 10.1016/0378-1119(85)90297-5

M3 - Article

C2 - 3891517

AN - SCOPUS:0021824586

SN - 0378-1119

VL - 34

SP - 81

EP - 86

JO - Gene

JF - Gene

IS - 1

ER -

Segments of chromosomal DNA from Rhynchosciara americana that undergo additional rounds of DNA replication in the salivary gland DNA puffs have only weak ARS activity in yeast

Abstract

Keywords

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