Screening essential genes of Mycobacterium tuberculosis with the pathway enrichment method

Guangyu Xu; Zhaohui Ni; Yue Shi; Xiaoyu Sun; Huaidong Wang; Chengguo Wei; Guoqing Wang; Fan Li

doi:10.1007/s11033-014-3654-z

Screening essential genes of Mycobacterium tuberculosis with the pathway enrichment method

Guangyu Xu, Zhaohui Ni, Yue Shi, Xiaoyu Sun, Huaidong Wang, Chengguo Wei, Guoqing Wang, Fan Li

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

The number of effective drugs for the prevention and control of tuberculosis is very limited. Therefore, high-throughput screening for Mycobacterium tuberculosis drug targets is critical. In addition, determining the essential gene cluster is important for both understanding a survival mechanism and finding novel molecular targets for anti-tuberculosis drugs. In this study, we applied the pathway enrichment method to perform high throughput screening of genes encoding key molecules for potential drug targets for M. tuberculosis. Our results indicated 122 genes that existed in more than three pathways, while four existed in 11 pathways. We predicted 55 genes that are potentially essential genes. Four of them, namely, Rv0363c, Rv0408, Rv0409 and Rv0794c, had the highest probability to be essential genes, and thus further experimental validation is warranted.

Original language	English
Pages (from-to)	7639-7644
Number of pages	6
Journal	Molecular Biology Reports
Volume	41
Issue number	11
DOIs	https://doi.org/10.1007/s11033-014-3654-z
State	Published - 21 Oct 2014
Externally published	Yes

Keywords

Essential gene
M. tuberculosis
Pathway

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10.1007/s11033-014-3654-z

Cite this

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abstract = "The number of effective drugs for the prevention and control of tuberculosis is very limited. Therefore, high-throughput screening for Mycobacterium tuberculosis drug targets is critical. In addition, determining the essential gene cluster is important for both understanding a survival mechanism and finding novel molecular targets for anti-tuberculosis drugs. In this study, we applied the pathway enrichment method to perform high throughput screening of genes encoding key molecules for potential drug targets for M. tuberculosis. Our results indicated 122 genes that existed in more than three pathways, while four existed in 11 pathways. We predicted 55 genes that are potentially essential genes. Four of them, namely, Rv0363c, Rv0408, Rv0409 and Rv0794c, had the highest probability to be essential genes, and thus further experimental validation is warranted.",

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author = "Guangyu Xu and Zhaohui Ni and Yue Shi and Xiaoyu Sun and Huaidong Wang and Chengguo Wei and Guoqing Wang and Fan Li",

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T1 - Screening essential genes of Mycobacterium tuberculosis with the pathway enrichment method

AU - Xu, Guangyu

AU - Ni, Zhaohui

AU - Shi, Yue

AU - Sun, Xiaoyu

AU - Wang, Huaidong

AU - Wei, Chengguo

AU - Wang, Guoqing

AU - Li, Fan

PY - 2014/10/21

Y1 - 2014/10/21

N2 - The number of effective drugs for the prevention and control of tuberculosis is very limited. Therefore, high-throughput screening for Mycobacterium tuberculosis drug targets is critical. In addition, determining the essential gene cluster is important for both understanding a survival mechanism and finding novel molecular targets for anti-tuberculosis drugs. In this study, we applied the pathway enrichment method to perform high throughput screening of genes encoding key molecules for potential drug targets for M. tuberculosis. Our results indicated 122 genes that existed in more than three pathways, while four existed in 11 pathways. We predicted 55 genes that are potentially essential genes. Four of them, namely, Rv0363c, Rv0408, Rv0409 and Rv0794c, had the highest probability to be essential genes, and thus further experimental validation is warranted.

AB - The number of effective drugs for the prevention and control of tuberculosis is very limited. Therefore, high-throughput screening for Mycobacterium tuberculosis drug targets is critical. In addition, determining the essential gene cluster is important for both understanding a survival mechanism and finding novel molecular targets for anti-tuberculosis drugs. In this study, we applied the pathway enrichment method to perform high throughput screening of genes encoding key molecules for potential drug targets for M. tuberculosis. Our results indicated 122 genes that existed in more than three pathways, while four existed in 11 pathways. We predicted 55 genes that are potentially essential genes. Four of them, namely, Rv0363c, Rv0408, Rv0409 and Rv0794c, had the highest probability to be essential genes, and thus further experimental validation is warranted.

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Screening essential genes of Mycobacterium tuberculosis with the pathway enrichment method

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Keywords

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