Screening essential genes of Mycobacterium tuberculosis with the pathway enrichment method

Guangyu Xu, Zhaohui Ni, Yue Shi, Xiaoyu Sun, Huaidong Wang, Chengguo Wei, Guoqing Wang, Fan Li

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

The number of effective drugs for the prevention and control of tuberculosis is very limited. Therefore, high-throughput screening for Mycobacterium tuberculosis drug targets is critical. In addition, determining the essential gene cluster is important for both understanding a survival mechanism and finding novel molecular targets for anti-tuberculosis drugs. In this study, we applied the pathway enrichment method to perform high throughput screening of genes encoding key molecules for potential drug targets for M. tuberculosis. Our results indicated 122 genes that existed in more than three pathways, while four existed in 11 pathways. We predicted 55 genes that are potentially essential genes. Four of them, namely, Rv0363c, Rv0408, Rv0409 and Rv0794c, had the highest probability to be essential genes, and thus further experimental validation is warranted.

Original languageEnglish
Pages (from-to)7639-7644
Number of pages6
JournalMolecular Biology Reports
Volume41
Issue number11
DOIs
StatePublished - 21 Oct 2014
Externally publishedYes

Keywords

  • Essential gene
  • M. tuberculosis
  • Pathway

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