@article{321a22736d7247dda789b0224d69a21b,
title = "SARS-CoV-2 mRNA vaccination induces functionally diverse antibodies to NTD, RBD, and S2",
abstract = "In this study we profiled vaccine-induced polyclonal antibodies as well as plasmablast-derived mAbs from individuals who received SARS-CoV-2 spike mRNA vaccine. Polyclonal antibody responses in vaccinees were robust and comparable to or exceeded those seen after natural infection. However, the ratio of binding to neutralizing antibodies after vaccination was greater than that after natural infection and, at the monoclonal level, we found that the majority of vaccine-induced antibodies did not have neutralizing activity. We also found a co-dominance of mAbs targeting the NTD and RBD of SARS-CoV-2 spike and an original antigenic-sin like backboost to spikes of seasonal human coronaviruses OC43 and HKU1. Neutralizing activity of NTD mAbs but not RBD mAbs against a clinical viral isolate carrying E484K as well as extensive changes in the NTD was abolished, suggesting that a proportion of vaccine-induced RBD binding antibodies may provide substantial protection against viral variants carrying single E484K RBD mutations.",
keywords = "NTD, RBD, SARS-CoV-2, mAbs, mRNA vaccination, plasmablasts, spike",
author = "{The Personalized Virology Initiative} and Fatima Amanat and Mahima Thapa and Tinting Lei and Ahmed, {Shaza M.Sayed} and Adelsberg, {Daniel C.} and Carre{\~n}o, {Juan Manuel} and Shirin Strohmeier and Schmitz, {Aaron J.} and Sarah Zafar and Zhou, {Julian Q.} and Willemijn Rijnink and Hala Alshammary and Nicholas Borcherding and Reiche, {Ana Gonzalez} and Komal Srivastava and Sordillo, {Emilia Mia} and {van Bakel}, Harm and Bulbul Ahmed and Deena Altman and Angela Amoako and Mahmoud Awawda and Katherine Beach and Carolina Berm{\'u}dez-Gonz{\'a}lez and Rachel Chernet and Lily Eaker and Shelcie Fabre and Ferreri, {Emily D.} and Daniel Floda and Charles Gleason and Giulio Kleiner and Denise Jurczyszak and Julia Matthews and Wanni Mendez and Mulder, {Lubbertus C.F.} and Jose Polanco and Kayla Russo and Ashley Salimbangon and Miti Saksena and Shin, {Amber S.} and Levy Sominsky and Sayahi Suthakaran and Ania Wajnberg and Turner, {Jackson S.} and Goran Bajic and Viviana Simon and Ellebedy, {Ali H.} and Florian Krammer",
note = "Publisher Copyright: {\textcopyright} 2021 Elsevier Inc.",
year = "2021",
month = jul,
day = "22",
doi = "10.1016/j.cell.2021.06.005",
language = "English",
volume = "184",
pages = "3936--3948.e10",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "15",
}