@article{7696aebff23748dd9fd367689f10eeb4,
title = "SARS-CoV-2 infects neurons and induces neuroinflammation in a non-human primate model of COVID-19",
abstract = "Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the etiologic agent of coronavirus disease 2019 (COVID-19), can induce a plethora of neurological complications in some patients. However, it is still under debate whether SARS-CoV-2 directly infects the brain or whether CNS sequelae result from systemic inflammatory responses triggered in the periphery. By using high-resolution microscopy, we investigated whether SARS-CoV-2 reaches the brain and how viral neurotropism can be modulated by aging in a non-human primate model of COVID-19. Seven days after infection, SARS-CoV-2 was detected in the olfactory cortex and interconnected regions and was accompanied by robust neuroinflammation and neuronal damage exacerbated in aged, diabetic animals. Our study provides an initial framework for identifying the molecular and cellular mechanisms underlying SARS-CoV-2 neurological complications, which will be essential to reducing both the short- and long-term burden of COVID-19.",
keywords = "CP: Microbiology, CP: Neuroscience, NHP, astrocytes, coronavirus, macaque, microglia, neurotropism, rhesus",
author = "Danielle Beckman and Alyssa Bonillas and Diniz, {Giovanne B.} and Sean Ott and Roh, {Jamin W.} and Elizaldi, {Sonny R.} and Schmidt, {Brian A.} and Sammak, {Rebecca L.} and {Van Rompay}, {Koen K.A.} and Iyer, {Smita S.} and Morrison, {John H.}",
note = "Funding Information: We would like to acknowledge the extensive contributions and knowledge shared by David Bennett, Wilhelm Von Morgenland, Miles Christensen, Vanessa Bakula, James Schulte, Jose Montoya, Joshua Holbrook, John McAnelly, Sadie Rogers, Christopher Nelson, Mark Allen, Dr. Lark Coffey, Hongwei Lu, Greg Hodge, Chelsea Schiano, and Nicole Drazenovich; Sheri Hild (ORIP, NIH) for assistance with funding acquisition; and Clint Florence (DMID/NIAID/NIH) and BEI Resources for providing the virus. Funding for this work was provided by the following agencies: National Institutes of Health grant 3RF1AG061001-01S1 (J.H.M. and S.S.I.); National Institutes of Health grant P51OD011107-59S6 (K.K.A.V.R., J.H.M., and S.S.I.); National Institutes of Health grant P51OD011107-61S2 (K.K.A.V.R., J.H.M., and S.S.I.); National Institutes of Health grant R21 AI143454-02S1 (S.S.I.); George Mason University FAST grant (S.S.I.); National Institutes of Health grant P51OD011107 (K.K.A.V.R., J.H.M., and S.S.I.); and National Institutes of Health grants National Institutes of Health P51OD011107-58S4 and P51OD011107-59S1. Funding Information: We would like to acknowledge the extensive contributions and knowledge shared by David Bennett, Wilhelm Von Morgenland, Miles Christensen, Vanessa Bakula, James Schulte, Jose Montoya, Joshua Holbrook, John McAnelly, Sadie Rogers, Christopher Nelson, Mark Allen, Dr. Lark Coffey, Hongwei Lu, Greg Hodge, Chelsea Schiano, and Nicole Drazenovich; Sheri Hild (ORIP, NIH) for assistance with funding acquisition; and Clint Florence (DMID/NIAID/NIH) and BEI Resources for providing the virus. Funding for this work was provided by the following agencies: National Institutes of Health grant 3RF1AG061001-01S1 (J.H.M. and S.S.I.); National Institutes of Health grant P51OD011107-59S6 (K.K.A.V.R. J.H.M. and S.S.I.); National Institutes of Health grant P51OD011107-61S2 (K.K.A.V.R. J.H.M. and S.S.I.); National Institutes of Health grant R21 AI143454-02S1 (S.S.I.); George Mason University FAST grant (S.S.I.); National Institutes of Health grant P51OD011107 (K.K.A.V.R. J.H.M. and S.S.I.); and National Institutes of Health grants National Institutes of Health P51OD011107-58S4 and P51OD011107-59S1. Conceptualization, S.S.I. and J.H.M.; investigation, D.B. A.B. G.B.D. S.O. J.W.R. S.R.E. B.A.S. R.L.S. K.K.A.V.R. S.S.I. and J.H.M.; project administration, K.K.A.V.R. S.S.I. and J.H.M.; supervision, J.H.M.; writing – original draft, D.B. G.B.D. and J.H.M.; writing – review & editing, D.B. G.B.D. K.K.A.V.R. S.S.I. and J.H.M. The authors declare that they have no competing interests. Publisher Copyright: {\textcopyright} 2022 The Author(s)",
year = "2022",
month = nov,
day = "1",
doi = "10.1016/j.celrep.2022.111573",
language = "English",
volume = "41",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "5",
}