Safety of P28GST, a protein derived from a schistosome helminth parasite, in patients with crohn’s disease: A pilot study (ACROHNEM)

Monique Capron, Laurent Béghin, Céline Leclercq, Julien Labreuche, Arnaud Dendooven, Annie Standaert, Marie Delbeke, Adeline Porcherie, Maria Nachury, Arnaud Boruchowicz, Jean Louis Dupas, Mathurin Fumery, Thierry Paupard, Sylviane Catteau, Dominique Deplanque, Jean Frederic Colombel, Pierre Desreumaux

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28 Scopus citations


Despite the development of novel therapies, inflammatory bowel diseases remain an innovative treatment challenge. Helminth therapy is a new promising approach, and a key issue is the identification of helminth-derived anti-inflammatory mediators. P28 glutathione-S-transferase (P28GST), a protein derived from schistosomes, a trematode parasitic helminth, was shown to reduce intestinal inflammation in experimental colitis by down-regulating the Th1/Th17 response. In this multicenter, open-label, pilot Phase 2a study, we evaluated the safety of P28GST administered to patients with mild Crohn’s disease (CD). We enrolled 10 patients with a baseline Crohn’s disease activity index (CDAI) value <220. Eight patients received two to three subcutaneous injections of recombinant P28GST with adjuvant. This three-month treatment was followed by a nine-month monitoring period. The primary endpoints were the monthly rate and seriousness of adverse events (AEs). Secondary endpoints were clinical recurrence, assessed with the CDAI as well as the levels of immunologic and inflammatory blood and tissue markers. The most common AEs were local or regional events at the injection site and gastrointestinal disorders. At three months after the first injection, CDAI scores and blood calprotectin levels decreased in parallel. These results indicate that P28GST showed promise as a safe and new therapeutic option for treating CD.

Original languageEnglish
Article number41
JournalJournal of Clinical Medicine
Issue number1
StatePublished - Jan 2020


  • Calprotectin
  • Crohn’s disease
  • Crohn’s disease activity index
  • Helminth protein
  • P28GST
  • Safety


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