TY - JOUR
T1 - Safe harbours for the integration of new DNA in the human genome
AU - Sadelain, Michel
AU - Papapetrou, Eirini P.
AU - Bushman, Frederic D.
N1 - Funding Information:
The authors thank N. Malani, S. Roth and A. Bailey for help with the Cancer Gene database. This work was supported by US NIH grants CA059350, HL053750, DK087923, AI052845 and AI082020 and by the New York State Stem Cell Science (NYSTEM) grant N08T060.
PY - 2012/1
Y1 - 2012/1
N2 - DNA and the host genome limit the reliability and safety of transgene integration for therapeutic cell engineering and other applications. Although targeted gene delivery has made considerable progress, the question of where to insert foreign sequences in the human genome to maximize safety and efficacy has received little attention. In this Opinion article, we discuss 'genomic safe harbours'-chromosomal locations where therapeutic transgenes can integrate and function in a predictable manner without perturbing endogenous gene activity and promoting cancer.
AB - DNA and the host genome limit the reliability and safety of transgene integration for therapeutic cell engineering and other applications. Although targeted gene delivery has made considerable progress, the question of where to insert foreign sequences in the human genome to maximize safety and efficacy has received little attention. In this Opinion article, we discuss 'genomic safe harbours'-chromosomal locations where therapeutic transgenes can integrate and function in a predictable manner without perturbing endogenous gene activity and promoting cancer.
UR - http://www.scopus.com/inward/record.url?scp=84655170273&partnerID=8YFLogxK
U2 - 10.1038/nrc3179
DO - 10.1038/nrc3179
M3 - Review article
C2 - 22129804
AN - SCOPUS:84655170273
SN - 1474-175X
VL - 12
SP - 51
EP - 58
JO - Nature Reviews Cancer
JF - Nature Reviews Cancer
IS - 1
ER -