S1P/S1P1 signaling stimulates cell migration and invasion in Wilms tumor

Mei Hong Li; Teresa Sanchez; Harold Yamase; Timothy Hla; Myat Lin Oo; Anna Pappalardo; Kevin R. Lynch; Chen Yong Lin; Fernando Ferrer

doi:10.1016/j.canlet.2008.11.025

S1P/S1P₁ signaling stimulates cell migration and invasion in Wilms tumor

Mei Hong Li, Teresa Sanchez, Harold Yamase, Timothy Hla, Myat Lin Oo, Anna Pappalardo, Kevin R. Lynch, Chen Yong Lin, Fernando Ferrer

Research output: Contribution to journal › Article › peer-review

51 Scopus citations

Abstract

Sphingosine-1-phosphate (S1P) is an important regulator of cellular functions via interaction with its receptors S1P_1-5. To date, nothing is known about the S1P receptor expression and the effects of S1P signaling in Wilms tumor. In this study, we found ubiquitous expression of S1P receptors in Wilms tumor specimens and cell lines. We demonstrated that S1P₁ acted as a promigratory modulator by employing S1P₁ antagonist VPC44116, S1P₁ siRNA and adenoviral transduction in Wilms tumor cells. Further, we clarified that S1P₁-mediated migration occurred via Gi coupling and activation of PI3K and Rac1. In addition, S1P stimulated WiT49 cell invasion through S1P₁/Gi signaling pathway. We consider that targeting S1P₁ may be a point of therapeutic intervention in Wilms tumor.

Original language	English
Pages (from-to)	171-179
Number of pages	9
Journal	Cancer Letters
Volume	276
Issue number	2
DOIs	https://doi.org/10.1016/j.canlet.2008.11.025
State	Published - 18 Apr 2009
Externally published	Yes

Keywords

Invasion
Migration
Sphingosine 1-phosphate
WiT49
Wilms tumor

Access to Document

10.1016/j.canlet.2008.11.025

Cite this

@article{aded8781ad26411ab871063e79ea4217,

title = "S1P/S1P1 signaling stimulates cell migration and invasion in Wilms tumor",

abstract = "Sphingosine-1-phosphate (S1P) is an important regulator of cellular functions via interaction with its receptors S1P1-5. To date, nothing is known about the S1P receptor expression and the effects of S1P signaling in Wilms tumor. In this study, we found ubiquitous expression of S1P receptors in Wilms tumor specimens and cell lines. We demonstrated that S1P1 acted as a promigratory modulator by employing S1P1 antagonist VPC44116, S1P1 siRNA and adenoviral transduction in Wilms tumor cells. Further, we clarified that S1P1-mediated migration occurred via Gi coupling and activation of PI3K and Rac1. In addition, S1P stimulated WiT49 cell invasion through S1P1/Gi signaling pathway. We consider that targeting S1P1 may be a point of therapeutic intervention in Wilms tumor.",

keywords = "Invasion, Migration, Sphingosine 1-phosphate, WiT49, Wilms tumor",

author = "Li, {Mei Hong} and Teresa Sanchez and Harold Yamase and Timothy Hla and Oo, {Myat Lin} and Anna Pappalardo and Lynch, {Kevin R.} and Lin, {Chen Yong} and Fernando Ferrer",

year = "2009",

month = apr,

day = "18",

doi = "10.1016/j.canlet.2008.11.025",

language = "English",

volume = "276",

pages = "171--179",

journal = "Cancer Letters",

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TY - JOUR

T1 - S1P/S1P1 signaling stimulates cell migration and invasion in Wilms tumor

AU - Li, Mei Hong

AU - Sanchez, Teresa

AU - Yamase, Harold

AU - Hla, Timothy

AU - Oo, Myat Lin

AU - Pappalardo, Anna

AU - Lynch, Kevin R.

AU - Lin, Chen Yong

AU - Ferrer, Fernando

PY - 2009/4/18

Y1 - 2009/4/18

N2 - Sphingosine-1-phosphate (S1P) is an important regulator of cellular functions via interaction with its receptors S1P1-5. To date, nothing is known about the S1P receptor expression and the effects of S1P signaling in Wilms tumor. In this study, we found ubiquitous expression of S1P receptors in Wilms tumor specimens and cell lines. We demonstrated that S1P1 acted as a promigratory modulator by employing S1P1 antagonist VPC44116, S1P1 siRNA and adenoviral transduction in Wilms tumor cells. Further, we clarified that S1P1-mediated migration occurred via Gi coupling and activation of PI3K and Rac1. In addition, S1P stimulated WiT49 cell invasion through S1P1/Gi signaling pathway. We consider that targeting S1P1 may be a point of therapeutic intervention in Wilms tumor.

AB - Sphingosine-1-phosphate (S1P) is an important regulator of cellular functions via interaction with its receptors S1P1-5. To date, nothing is known about the S1P receptor expression and the effects of S1P signaling in Wilms tumor. In this study, we found ubiquitous expression of S1P receptors in Wilms tumor specimens and cell lines. We demonstrated that S1P1 acted as a promigratory modulator by employing S1P1 antagonist VPC44116, S1P1 siRNA and adenoviral transduction in Wilms tumor cells. Further, we clarified that S1P1-mediated migration occurred via Gi coupling and activation of PI3K and Rac1. In addition, S1P stimulated WiT49 cell invasion through S1P1/Gi signaling pathway. We consider that targeting S1P1 may be a point of therapeutic intervention in Wilms tumor.

KW - Invasion

KW - Migration

KW - Sphingosine 1-phosphate

KW - WiT49

KW - Wilms tumor

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