TY - JOUR
T1 - RSC, an essential, abundant chromatin-remodeling complex
AU - Cairns, Bradley R.
AU - Lorch, Yahli
AU - Li, Yang
AU - Zhang, Mincheng
AU - Lacomis, Lynne
AU - Erdjument-Bromage, Hediye
AU - Tempst, Paul
AU - Du, Jian
AU - Laurent, Brehon
AU - Kornberg, Roger D.
N1 - Funding Information:
The authors wish to thank Fred Winston for support of B. R. C. during the completion of this work, Dr. Matthias Mann for use of the PeptideSearch algorithm and the most recent NRDB IndexFile, and Y.-J. Kim for anti-Swi3 antibodies. We also thank Grant Hartzog, Fred Winston, and Tom Owen-Hughes for their comments on the manuscript. Supported by grants from the National Institutes of Health (GM36659) and the March of Dimes (to R. D. K.); by National Science Foundation grant BIR-9420123 (to P. T.); by National Cancer Institute Core Grant 5 P30 CA08748 (to the Memorial Sloan-Kettering Cancer Center Sequencing Laboratory); and by American Cancer Society grant NP-871 and a Basil O'Connor Starter Scholar Research Award from the March of Dimes (to B. L.). Y. Li. was supported by a Bank of America-Giannini Foundation Fellowship for medical research in California. B. R. C. is a fellow of the Leukemia Society of America.
PY - 1996/12/27
Y1 - 1996/12/27
N2 - A novel 15-subunit complex with the capacity to remodel the structure of chromatin, termed RSC, has been isolated from S. cerevisiae on the basis of homology to the SWI/SNF complex. At least three RSC subunits are related to SWI/SNF polypeptides: Sth1p, Rsc6p, and Rsc8p are significantly similar to Swi2/Snf2p, Swp73p, and Swi3p, respectively, and were identified by mass spectrometric and sequence analysis of peptide fragments. Like SWI/SNF, RSC exhibits a DNA-dependent ATPase activity stimulated by both free and nucleosomal DNA and a capacity to perturb nucleosome structure. RSC is, however, at least 10-fold more abundant than SWI/SNF complex and is essential for mitotic growth. Contrary to a report for SWI/SNF complex, no association of RSC (nor of SWI/SNF complex) with RNA polymerase II holoenzyme was detected.
AB - A novel 15-subunit complex with the capacity to remodel the structure of chromatin, termed RSC, has been isolated from S. cerevisiae on the basis of homology to the SWI/SNF complex. At least three RSC subunits are related to SWI/SNF polypeptides: Sth1p, Rsc6p, and Rsc8p are significantly similar to Swi2/Snf2p, Swp73p, and Swi3p, respectively, and were identified by mass spectrometric and sequence analysis of peptide fragments. Like SWI/SNF, RSC exhibits a DNA-dependent ATPase activity stimulated by both free and nucleosomal DNA and a capacity to perturb nucleosome structure. RSC is, however, at least 10-fold more abundant than SWI/SNF complex and is essential for mitotic growth. Contrary to a report for SWI/SNF complex, no association of RSC (nor of SWI/SNF complex) with RNA polymerase II holoenzyme was detected.
UR - http://www.scopus.com/inward/record.url?scp=0030447612&partnerID=8YFLogxK
U2 - 10.1016/S0092-8674(00)81820-6
DO - 10.1016/S0092-8674(00)81820-6
M3 - Article
C2 - 8980231
AN - SCOPUS:0030447612
SN - 0092-8674
VL - 87
SP - 1249
EP - 1260
JO - Cell
JF - Cell
IS - 7
ER -