RSC, an essential, abundant chromatin-remodeling complex

Bradley R. Cairns, Yahli Lorch, Yang Li, Mincheng Zhang, Lynne Lacomis, Hediye Erdjument-Bromage, Paul Tempst, Jian Du, Brehon Laurent, Roger D. Kornberg

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597 Scopus citations

Abstract

A novel 15-subunit complex with the capacity to remodel the structure of chromatin, termed RSC, has been isolated from S. cerevisiae on the basis of homology to the SWI/SNF complex. At least three RSC subunits are related to SWI/SNF polypeptides: Sth1p, Rsc6p, and Rsc8p are significantly similar to Swi2/Snf2p, Swp73p, and Swi3p, respectively, and were identified by mass spectrometric and sequence analysis of peptide fragments. Like SWI/SNF, RSC exhibits a DNA-dependent ATPase activity stimulated by both free and nucleosomal DNA and a capacity to perturb nucleosome structure. RSC is, however, at least 10-fold more abundant than SWI/SNF complex and is essential for mitotic growth. Contrary to a report for SWI/SNF complex, no association of RSC (nor of SWI/SNF complex) with RNA polymerase II holoenzyme was detected.

Original languageEnglish
Pages (from-to)1249-1260
Number of pages12
JournalCell
Volume87
Issue number7
DOIs
StatePublished - 27 Dec 1996
Externally publishedYes

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