TY - JOUR
T1 - Rpb4 and Puf3 imprint and post-transcriptionally control the stability of a common set of mRNAs in yeast
AU - Garrido-Godino, A. I.
AU - Gupta, I.
AU - Gutiérrez-Santiago, F.
AU - Martínez-Padilla, A. B.
AU - Alekseenko, A.
AU - Steinmetz, L. M.
AU - Pérez-Ortín, J. E.
AU - Pelechano, V.
AU - Navarro, F.
N1 - Publisher Copyright:
© 2020 Informa UK Limited, trading as Taylor & Francis Group.
PY - 2021
Y1 - 2021
N2 - Gene expression involving RNA polymerase II is regulated by the concerted interplay between mRNA synthesis and degradation, crosstalk in which mRNA decay machinery and transcription machinery respectively impact transcription and mRNA stability. Rpb4, and likely dimer Rpb4/7, seem the central components of the RNA pol II governing these processes. In this work we unravel the molecular mechanisms participated by Rpb4 that mediate the posttranscriptional events regulating mRNA imprinting and stability. By RIP-Seq, we analysed genome-wide the association of Rpb4 with mRNAs and demonstrated that it targeted a large population of more than 1400 transcripts. A group of these mRNAs was also the target of the RNA binding protein, Puf3. We demonstrated that Rpb4 and Puf3 physically, genetically, and functionally interact and also affect mRNA stability, and likely the imprinting, of a common group of mRNAs. Furthermore, the Rpb4 and Puf3 association with mRNAs depends on one another. We also demonstrated, for the first time, that Puf3 associates with chromatin in an Rpb4-dependent manner. Our data also suggest that Rpb4 could be a key element of the RNA pol II that coordinates mRNA synthesis, imprinting and stability in cooperation with RBPs.
AB - Gene expression involving RNA polymerase II is regulated by the concerted interplay between mRNA synthesis and degradation, crosstalk in which mRNA decay machinery and transcription machinery respectively impact transcription and mRNA stability. Rpb4, and likely dimer Rpb4/7, seem the central components of the RNA pol II governing these processes. In this work we unravel the molecular mechanisms participated by Rpb4 that mediate the posttranscriptional events regulating mRNA imprinting and stability. By RIP-Seq, we analysed genome-wide the association of Rpb4 with mRNAs and demonstrated that it targeted a large population of more than 1400 transcripts. A group of these mRNAs was also the target of the RNA binding protein, Puf3. We demonstrated that Rpb4 and Puf3 physically, genetically, and functionally interact and also affect mRNA stability, and likely the imprinting, of a common group of mRNAs. Furthermore, the Rpb4 and Puf3 association with mRNAs depends on one another. We also demonstrated, for the first time, that Puf3 associates with chromatin in an Rpb4-dependent manner. Our data also suggest that Rpb4 could be a key element of the RNA pol II that coordinates mRNA synthesis, imprinting and stability in cooperation with RBPs.
KW - Puf3
KW - RNA polymerase II
KW - Rbp
KW - Saccharomyces cerevisiae
KW - mRNA decay
KW - mRNA imprinting
UR - http://www.scopus.com/inward/record.url?scp=85094888425&partnerID=8YFLogxK
U2 - 10.1080/15476286.2020.1839229
DO - 10.1080/15476286.2020.1839229
M3 - Article
C2 - 33094674
AN - SCOPUS:85094888425
SN - 1547-6286
VL - 18
SP - 1206
EP - 1220
JO - RNA Biology
JF - RNA Biology
IS - 8
ER -