Role of poly(ADP-ribose) synthetase activation in the development of experimental allergic encephalomyelitis

G. S. Scott; P. Hake; R. B. Kean; L. Virág; C. Szabó; D. C. Hooper

doi:10.1016/S0165-5728(01)00329-0

Role of poly(ADP-ribose) synthetase activation in the development of experimental allergic encephalomyelitis

G. S. Scott, P. Hake, R. B. Kean, L. Virág, C. Szabó, D. C. Hooper

Research output: Contribution to journal › Article › peer-review

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Abstract

Peroxynitrite formation has been demonstrated during experimental allergic encephalomyelitis (EAE). Furthermore, peroxynitrite has been identified as an activator of poly(ADP-ribose) synthetase (PARS), an enzyme implicated in neurotoxicity. In the current study, we examined the role of PARS activation in the development of EAE. Administration of the PARS inhibitor 5-iodo-6-amino-1,2-benzopyrone (INH₂BP) delayed the onset of EAE and reduced the incidence and severity of disease signs. Moreover, drug treatment lowered iNOS activity and decreased cell infiltration in cervical spinal tissues from EAE-sensitized animals. To conclude, the results of the present investigation suggest that PARS activity may contribute to the pathogenesis of EAE.

Original language	English
Pages (from-to)	78-86
Number of pages	9
Journal	Journal of Neuroimmunology
Volume	117
Issue number	1-2
DOIs	https://doi.org/10.1016/S0165-5728(01)00329-0
State	Published - 2 Jul 2001
Externally published	Yes

Keywords

Blood-brain barrier
Experimental allergic encephalomyelitis
Inflammation
Peroxynitrite
Poly(ADP-ribose) synthetase

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10.1016/S0165-5728(01)00329-0

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title = "Role of poly(ADP-ribose) synthetase activation in the development of experimental allergic encephalomyelitis",

abstract = "Peroxynitrite formation has been demonstrated during experimental allergic encephalomyelitis (EAE). Furthermore, peroxynitrite has been identified as an activator of poly(ADP-ribose) synthetase (PARS), an enzyme implicated in neurotoxicity. In the current study, we examined the role of PARS activation in the development of EAE. Administration of the PARS inhibitor 5-iodo-6-amino-1,2-benzopyrone (INH2BP) delayed the onset of EAE and reduced the incidence and severity of disease signs. Moreover, drug treatment lowered iNOS activity and decreased cell infiltration in cervical spinal tissues from EAE-sensitized animals. To conclude, the results of the present investigation suggest that PARS activity may contribute to the pathogenesis of EAE.",

keywords = "Blood-brain barrier, Experimental allergic encephalomyelitis, Inflammation, Peroxynitrite, Poly(ADP-ribose) synthetase",

author = "Scott, {G. S.} and P. Hake and Kean, {R. B.} and L. Vir{\'a}g and C. Szab{\'o} and Hooper, {D. C.}",

note = "Funding Information: The assistance of Mr. Michael O'Conor with the iNOS activity measurements is greatly appreciated. This work was supported, in part, by a grant from Octamer (Mill Valley, CA) (to C.S. and G.S.S.) and by National Multiple Sclerosis Society grant RG2896A8/5 (to D.C.H.). G.S.S. was supported by a grant from the Paralyzed Veterans of America Spinal Cord Research Foundation.",

year = "2001",

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doi = "10.1016/S0165-5728(01)00329-0",

language = "English",

volume = "117",

pages = "78--86",

journal = "Journal of Neuroimmunology",

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T1 - Role of poly(ADP-ribose) synthetase activation in the development of experimental allergic encephalomyelitis

AU - Scott, G. S.

AU - Hake, P.

AU - Kean, R. B.

AU - Virág, L.

AU - Szabó, C.

AU - Hooper, D. C.

N1 - Funding Information: The assistance of Mr. Michael O'Conor with the iNOS activity measurements is greatly appreciated. This work was supported, in part, by a grant from Octamer (Mill Valley, CA) (to C.S. and G.S.S.) and by National Multiple Sclerosis Society grant RG2896A8/5 (to D.C.H.). G.S.S. was supported by a grant from the Paralyzed Veterans of America Spinal Cord Research Foundation.

PY - 2001/7/2

Y1 - 2001/7/2

N2 - Peroxynitrite formation has been demonstrated during experimental allergic encephalomyelitis (EAE). Furthermore, peroxynitrite has been identified as an activator of poly(ADP-ribose) synthetase (PARS), an enzyme implicated in neurotoxicity. In the current study, we examined the role of PARS activation in the development of EAE. Administration of the PARS inhibitor 5-iodo-6-amino-1,2-benzopyrone (INH2BP) delayed the onset of EAE and reduced the incidence and severity of disease signs. Moreover, drug treatment lowered iNOS activity and decreased cell infiltration in cervical spinal tissues from EAE-sensitized animals. To conclude, the results of the present investigation suggest that PARS activity may contribute to the pathogenesis of EAE.

AB - Peroxynitrite formation has been demonstrated during experimental allergic encephalomyelitis (EAE). Furthermore, peroxynitrite has been identified as an activator of poly(ADP-ribose) synthetase (PARS), an enzyme implicated in neurotoxicity. In the current study, we examined the role of PARS activation in the development of EAE. Administration of the PARS inhibitor 5-iodo-6-amino-1,2-benzopyrone (INH2BP) delayed the onset of EAE and reduced the incidence and severity of disease signs. Moreover, drug treatment lowered iNOS activity and decreased cell infiltration in cervical spinal tissues from EAE-sensitized animals. To conclude, the results of the present investigation suggest that PARS activity may contribute to the pathogenesis of EAE.

KW - Blood-brain barrier

KW - Experimental allergic encephalomyelitis

KW - Inflammation

KW - Peroxynitrite

KW - Poly(ADP-ribose) synthetase

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AN - SCOPUS:0035796895

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IS - 1-2

ER -

Role of poly(ADP-ribose) synthetase activation in the development of experimental allergic encephalomyelitis

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Keywords

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