ROLE OF LYSOSOMES IN THE LYMPHOCYTE RESPONSE

TY - JOUR

T1 - ROLE OF LYSOSOMES IN THE LYMPHOCYTE RESPONSE

AU - Hirschhorn, Kurt

AU - Hirschhorn, Rochelle

N1 - Funding Information: 2-7 mg. per 100 ml. is the same in patients taking 16 mg. magnesium per day in the form of oxo, whether given intravenous therapy initially or not. Recent observations on a few patients suggest that high dosage of oxo results in a more rapid rise of plasma-magnesium levels. Four teaspoons of oxo daily will maintain a level of about 2-7 mg. per 100 ml. in most of the patients studied, though a few require more and some a lesser amount. It is of interest that the plasma level rises and remains raised by such a small daily supplement of magnesium. These preliminary observations suggest that the plasma- magnesium levels of men with myocardial infarction are lower than in healthy men of comparable age. The deficiency may be replaced easily by taking oxo. In a small group of women with myocardial infarction, only a third showed a lower plasma-magnesium level. Thus whilst relative plasma-magnesium deficiency may play a part in the xtiology of myocardial infarction, it is unlikely to be the only factor. This work continues. We. are grateful to the Welsh Hospital Board and the North Monmouthshire hospital management committee for financial assistance; Dr. H. Campbell for the statistical analysis of the results; to Mr. P. 0. Dennis, chief chemist, Oxo Ltd., for his advice; and to Prof. F. W. Landgrebe and Dr. William Phillips. The plasma- magnesium and plasma-calcium levels were determined by Mr. A. J. L. Wycherley, F.I.M.L.T., without whose generous help this Funding Information: be the release of hydrolytic enzymes during the endocytosis of such macromolecular complexes. During the remainder of the culture period, as the cell enlarges, there is an increase in the number of lysosomes and in the concentration of lysosomal enzymes. 1 8 10 This continues until the second or premitotic phase. Preceding and during the early stages of mitosis, the new lysosome- like structures aggregate at the nuclear membrane 22 23 and later at the spindle. 24 These two structures must disintegrate for proper cell-division and reconstruction of the daughter nuclei to occur. Allison has described diminution of visible lysosomes at the time of mitosis. Possibly a controlled release of lysosomal enzymes is required for the destruction of those structures which disappear during the mitotic process. SUMMARY We have presented a hypothesis which proposes that the release of lysosomal enzymes is an essential factor in the rebuilding processes involved in cellular " derepres- sion " and mitosis. In the former these enzymes would produce breakdown of essential precursors, while in the latter they would destroy the structures which must dis- appear if cells are to divide normally. The experimental studies previous to this hypothesis were sup- ported by grants from the U.S. Public Health Service (HD-00542), the American Heart Association (K. H. Established Investigator), the New York City Health Research Council (U-1030), and the Association for the Aid of Crippled Children. KURT HIRSCHHORN M.D. New York ROCHELLE HIRSCHHORN M.D. New York

PY - 1965/5/15

Y1 - 1965/5/15

UR - https://www.scopus.com/pages/publications/26844552309

U2 - 10.1016/S0140-6736(65)91318-8

DO - 10.1016/S0140-6736(65)91318-8

M3 - Article

C2 - 14283743

AN - SCOPUS:26844552309

SN - 0140-6736

VL - 285

SP - 1046

EP - 1047

JO - The Lancet

JF - The Lancet

IS - 7394

ER -