Role of LpL (Lipoprotein Lipase) in macrophage polarization in vitro and in vivo

Hye Rim Chang, Tatjana Josefs, Diego Scerbo, Namrata Gumaste, Yunying Hu, Lesley Ann Huggins, Tessa J. Barrett, Stephanie S. Chiang, Jennifer Grossman, Svetlana Bagdasarov, Edward A. Fisher, Ira J. Goldberg

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Objective: Fatty acid uptake and oxidation characterize the metabolism of alternatively activated macrophage polarization in vitro, but the in vivo biology is less clear. We assessed the roles of LpL (lipoprotein lipase)-mediated lipid uptake in macrophage polarization in vitro and in several important tissues in vivo. Approach and Results: We created mice with both global and myeloid-cell specific LpL deficiency. LpL deficiency in the presence of VLDL (very low-density lipoproteins) altered gene expression of bone marrow-derived macrophages and led to reduced lipid uptake but an increase in some anti- and some proinflammatory markers. However, LpL deficiency did not alter lipid accumulation or gene expression in circulating monocytes nor did it change the ratio of Ly6Chigh/Ly6Clow. In adipose tissue, less macrophage lipid accumulation was found with global but not myeloid-specific LpL deficiency. Neither deletion affected the expression of inflammatory genes. Global LpL deficiency also reduced the numbers of elicited peritoneal macrophages. Finally, we assessed gene expression in macrophages from atherosclerotic lesions during regression; LpL deficiency did not affect the polarity of plaque macrophages. Conclusions: The phenotypic changes observed in macrophages upon deletion of Lpl in vitro is not mimicked in tissue macrophages.

Original languageEnglish
Pages (from-to)1967-1985
Number of pages19
JournalArteriosclerosis, Thrombosis, and Vascular Biology
Volume39
Issue number10
DOIs
StatePublished - 1 Oct 2019
Externally publishedYes

Keywords

  • Fatty acid
  • Inflammation
  • Lipids
  • Macrophage
  • Monocytes

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