TY - JOUR
T1 - Role of interleukin 28B rs12979860 C/T polymorphism on the histological outcome of chronic hepatitis C
T2 - Relationship with gender and viral genotype
AU - Falleti, Edmondo
AU - Bitetto, Davide
AU - Fabris, Carlo
AU - Cussigh, Annarosa
AU - Fornasiere, Ezio
AU - Cmet, Sara
AU - Fumolo, Elisa
AU - Bignulin, Sara
AU - Fontanini, Elisabetta
AU - Cerutti, Andrea
AU - Minisini, Rosalba
AU - Pirisi, Mario
AU - Toniutto, Pierluigi
N1 - Funding Information:
Funding This study was supported in part by grants from the “Ricerca Sanitaria Finalizzata” Program, Regione Piemonte, Italy and Fondazione CRUP Udine, Italy.
PY - 2011/10
Y1 - 2011/10
N2 - Background This study aimed to determine whether the single-nucleotide polymorphism (rs12979860 C/T) of the interleukin 28B (IL-28B) gene, which is associated with hepatitis C virus (HCV) clearance, is also associated with fibrosis in chronic HCV infection. Methods An RFLP-PCR technique was used to genotype 629 HCV-positive patients (200 with cirrhosis) and 428 healthy control subjects. Results The genotype frequencies in the controls and chronic hepatitis C patients were as follows: C/C 47.0% vs. 32.6%, C/T 41.8% vs. 52.8% and T/T 11.2% vs. 14.6% (p<0.0001). The C allele frequency was higher in HCV-2- (0.635) and 3- (0.692) infected patients in comparison to those infected with HCV-1 (0.550) or 4-5 (0.600) (p<0.001). Infected T/T homozygotes had a mean staging score higher than other patients (3.50 vs. 3.04, p<0.05). Conclusions IL-28B rs12979860 C/T polymorphism is associated with a greater likelihood of HCV persistence, particularly in HCV genotypes 1 and 4. The T allele affects the severity of liver fibrosis.
AB - Background This study aimed to determine whether the single-nucleotide polymorphism (rs12979860 C/T) of the interleukin 28B (IL-28B) gene, which is associated with hepatitis C virus (HCV) clearance, is also associated with fibrosis in chronic HCV infection. Methods An RFLP-PCR technique was used to genotype 629 HCV-positive patients (200 with cirrhosis) and 428 healthy control subjects. Results The genotype frequencies in the controls and chronic hepatitis C patients were as follows: C/C 47.0% vs. 32.6%, C/T 41.8% vs. 52.8% and T/T 11.2% vs. 14.6% (p<0.0001). The C allele frequency was higher in HCV-2- (0.635) and 3- (0.692) infected patients in comparison to those infected with HCV-1 (0.550) or 4-5 (0.600) (p<0.001). Infected T/T homozygotes had a mean staging score higher than other patients (3.50 vs. 3.04, p<0.05). Conclusions IL-28B rs12979860 C/T polymorphism is associated with a greater likelihood of HCV persistence, particularly in HCV genotypes 1 and 4. The T allele affects the severity of liver fibrosis.
KW - Interleukin 28B polymorphism
KW - gender
KW - hepatitis C virus
KW - liver fibrosis
KW - viral genotypes
UR - http://www.scopus.com/inward/record.url?scp=80755140137&partnerID=8YFLogxK
U2 - 10.1007/s10875-011-9547-1
DO - 10.1007/s10875-011-9547-1
M3 - Article
C2 - 21647799
AN - SCOPUS:80755140137
SN - 0271-9142
VL - 31
SP - 891
EP - 899
JO - Journal of Clinical Immunology
JF - Journal of Clinical Immunology
IS - 5
ER -