Role of Immunoglobulin M and A Antibodies in the Neutralization of Severe Acute Respiratory Syndrome Coronavirus 2

JÃromine Klingler, Svenja Weiss, Vincenza Itri, Xiaomei Liu, Kasopefoluwa Y. Oguntuyo, Christian Stevens, Satoshi Ikegame, Chuan Tien Hung, Gospel Enyindah-Asonye, Fatima Amanat, Ian Baine, Suzanne Arinsburg, Juan C. Bandres, Erna Milunka Kojic, Jonathan Stoever, Denise Jurczyszak, Maria Bermudez-Gonzalez, Arthur NÃidas, Sean Liu, Benhur LeeSusan Zolla-Pazner, Catarina E. Hioe

Research output: Contribution to journalArticlepeer-review

31 Scopus citations


Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has infected millions of people globally. Virus infection requires the receptor-binding domain (RBD) of the spike protein. Although studies have demonstrated anti-spike and-RBD antibodies to be protective in animal models, and convalescent plasma as a promising therapeutic option, little is known about immunoglobulin isotypes capable of blocking infection. Methods: We studied spike- A nd RBD-specific immunoglobulin isotypes in convalescent and acute plasma/serum samples using a multiplex bead assay. We also determined virus neutralization activities in plasma and serum samples, and purified immunoglobulin fractions using a vesicular stomatitis pseudovirus assay. Results: Spike- A nd RBD-specific immunoglobulin (Ig) M, IgG1, and IgA1 were produced by all or nearly all subjects at variable levels and detected early after infection. All samples displayed neutralizing activity. Regression analyses revealed that IgM and IgG1 contributed most to neutralization, consistent with IgM and IgG fractions' neutralization potency. IgA also exhibited neutralizing activity, but with lower potency. Conclusion: IgG, IgM, and IgA are critical components of convalescent plasma used for treatment of coronavirus disease 2019 (COVID-19).

Original languageEnglish
Pages (from-to)957-970
Number of pages14
JournalJournal of Infectious Diseases
Issue number6
StatePublished - 15 Mar 2021


  • COVID-19
  • SARS-CoV-2
  • antibody isotypes
  • convalescent plasma
  • neutralization


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