Role of Chromatin assembly factor 1 in DNA replication of Plasmodium falciparum

Mohit Kumar Gupta; Meetu Agarawal; Khadija Banu; K. Sony Reddy; Deepak Gaur; Suman Kumar Dhar

doi:10.1016/j.bbrc.2017.11.131

Role of Chromatin assembly factor 1 in DNA replication of Plasmodium falciparum

Mohit Kumar Gupta, Meetu Agarawal, Khadija Banu, K. Sony Reddy, Deepak Gaur, Suman Kumar Dhar

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7 Scopus citations

Abstract

Nucleosome assembly in P. falciparum could be the key process in maintaining its genomic integrity as DNA replicates more than once per cell cycle during several stages of its life cycle. Here, we report the functional characterization of P. falciparum chromatin assembly factor 1 (CAF1), which interacts with several proteins namely PfCAF2, Histones, PfHP1 and others. Consistent with the above findings, we demonstrate the presence of PfCAF1 at the telomeric repeat regions, central and subtelomeric var genes of multiple var gene family along with PfHP1. Further, we report the upregulation of PfCAF1 after treatment with genotoxic agents like MMS and HU. Together, these findings establish role of PfCAF1 in heterochromatin maintenance and as histone chaperone in nucleosome assembly and DNA damage repair.

Original language	English
Pages (from-to)	1285-1291
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	495
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2017.11.131
State	Published - 1 Jan 2018
Externally published	Yes

Keywords

CAF1
Chaperone
Heterochromatin
Malaria
PfHP1
Replication

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10.1016/j.bbrc.2017.11.131

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title = "Role of Chromatin assembly factor 1 in DNA replication of Plasmodium falciparum",

abstract = "Nucleosome assembly in P. falciparum could be the key process in maintaining its genomic integrity as DNA replicates more than once per cell cycle during several stages of its life cycle. Here, we report the functional characterization of P. falciparum chromatin assembly factor 1 (CAF1), which interacts with several proteins namely PfCAF2, Histones, PfHP1 and others. Consistent with the above findings, we demonstrate the presence of PfCAF1 at the telomeric repeat regions, central and subtelomeric var genes of multiple var gene family along with PfHP1. Further, we report the upregulation of PfCAF1 after treatment with genotoxic agents like MMS and HU. Together, these findings establish role of PfCAF1 in heterochromatin maintenance and as histone chaperone in nucleosome assembly and DNA damage repair.",

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AU - Gupta, Mohit Kumar

AU - Agarawal, Meetu

AU - Banu, Khadija

AU - Reddy, K. Sony

AU - Gaur, Deepak

AU - Dhar, Suman Kumar

PY - 2018/1/1

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N2 - Nucleosome assembly in P. falciparum could be the key process in maintaining its genomic integrity as DNA replicates more than once per cell cycle during several stages of its life cycle. Here, we report the functional characterization of P. falciparum chromatin assembly factor 1 (CAF1), which interacts with several proteins namely PfCAF2, Histones, PfHP1 and others. Consistent with the above findings, we demonstrate the presence of PfCAF1 at the telomeric repeat regions, central and subtelomeric var genes of multiple var gene family along with PfHP1. Further, we report the upregulation of PfCAF1 after treatment with genotoxic agents like MMS and HU. Together, these findings establish role of PfCAF1 in heterochromatin maintenance and as histone chaperone in nucleosome assembly and DNA damage repair.

AB - Nucleosome assembly in P. falciparum could be the key process in maintaining its genomic integrity as DNA replicates more than once per cell cycle during several stages of its life cycle. Here, we report the functional characterization of P. falciparum chromatin assembly factor 1 (CAF1), which interacts with several proteins namely PfCAF2, Histones, PfHP1 and others. Consistent with the above findings, we demonstrate the presence of PfCAF1 at the telomeric repeat regions, central and subtelomeric var genes of multiple var gene family along with PfHP1. Further, we report the upregulation of PfCAF1 after treatment with genotoxic agents like MMS and HU. Together, these findings establish role of PfCAF1 in heterochromatin maintenance and as histone chaperone in nucleosome assembly and DNA damage repair.

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KW - Chaperone

KW - Heterochromatin

KW - Malaria

KW - PfHP1

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Role of Chromatin assembly factor 1 in DNA replication of Plasmodium falciparum

Abstract

Keywords

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