TY - JOUR
T1 - Role of CCR8 and other chemokine pathways in the migration of monocyte-derived dendritic cells to lymph nodes
AU - Qu, Chunfeng
AU - Edwards, Emmerson W.
AU - Tacke, Frank
AU - Angeli, Véronique
AU - Llodrá, Jaime
AU - Sanchez-Schmitz, Guzman
AU - Garin, Alexandre
AU - Haque, Nasreen S.
AU - Peters, Wendy
AU - Van Rooijen, Nico
AU - Sanchez-Torres, Carmen
AU - Bromberg, Jonathan
AU - Charo, Israel F.
AU - Jung, Steffen
AU - Lira, Sergio A.
AU - Randolph, Gwendalyn J.
PY - 2004/11/15
Y1 - 2004/11/15
N2 - Studying the influence of chemokine receptors (CCRs) on monocyte fate may reveal information about which subpopulations of monocytes convert to dendritic cells (DCs) and the migration pathways that they use. First, we examined whether prominent CCRs on different monocyte subsets, CCR2 or CX3CR1, mediated migration events upstream of the accumulation of monocyte-derived DCs in lymph nodes (LNs). Monocytes were labeled and traced by uptake of latex microspheres in skin. Unexpectedly, neither CCR2 nor CX3CR1 were required. However, absence of CCR2 led to an increased labeling of the minor Gr-1int monocyte population, and the number of latex+ DCs that emigrated to LNs was correspondingly increased. Characterization of Gr-1int monocytes revealed that they selectively expressed CCR7 and CCR8 mRNA in blood. CCR7 and CCR8 pathways were used by monocyte-derived DCs during mobilization from skin to LNs. The role of CCR8 in emigration from tissues also applied to human monocyte-derived cells in a model of transendothelial trafficking. Collectively, the data suggest that Gr-1 int monocytes may be most disposed to become a lymphatic-migrating DCs. When these monocyte-derived DCs exit skin to emigrate to LNs, they use not only CCR7 but also CCR8, which was not previously recognized to participate in migration to LNs.
AB - Studying the influence of chemokine receptors (CCRs) on monocyte fate may reveal information about which subpopulations of monocytes convert to dendritic cells (DCs) and the migration pathways that they use. First, we examined whether prominent CCRs on different monocyte subsets, CCR2 or CX3CR1, mediated migration events upstream of the accumulation of monocyte-derived DCs in lymph nodes (LNs). Monocytes were labeled and traced by uptake of latex microspheres in skin. Unexpectedly, neither CCR2 nor CX3CR1 were required. However, absence of CCR2 led to an increased labeling of the minor Gr-1int monocyte population, and the number of latex+ DCs that emigrated to LNs was correspondingly increased. Characterization of Gr-1int monocytes revealed that they selectively expressed CCR7 and CCR8 mRNA in blood. CCR7 and CCR8 pathways were used by monocyte-derived DCs during mobilization from skin to LNs. The role of CCR8 in emigration from tissues also applied to human monocyte-derived cells in a model of transendothelial trafficking. Collectively, the data suggest that Gr-1 int monocytes may be most disposed to become a lymphatic-migrating DCs. When these monocyte-derived DCs exit skin to emigrate to LNs, they use not only CCR7 but also CCR8, which was not previously recognized to participate in migration to LNs.
KW - Chemotaxis
KW - Endothelium
KW - Inflammation
KW - Lymphatic system
KW - Macrophage
UR - http://www.scopus.com/inward/record.url?scp=9244240285&partnerID=8YFLogxK
U2 - 10.1084/jem.20032152
DO - 10.1084/jem.20032152
M3 - Article
C2 - 15534368
AN - SCOPUS:9244240285
SN - 0022-1007
VL - 200
SP - 1231
EP - 1241
JO - Journal of Experimental Medicine
JF - Journal of Experimental Medicine
IS - 10
ER -