Role of αβ receptor heterodimer formation in β platelet-derived growth factor (PDGF) receptor activation by PDGF-AB

Mohammad A. Heidaran, Jacalyn H. Pierce, Jin Chen Yu, Daniela Lombardi, Jennifer E. Artrip, Timothy P. Fleming, Arlen Thomason, Stuart A. Aaronson

Research output: Contribution to journalArticlepeer-review

75 Scopus citations

Abstract

We investigated the ability of highly purified recombinant platelet-derived growth factor (PDGF) AB to interact with the products of α and β receptor genes expressed in cells independently or concurrently. Although PDGF-AB lacked any detectable ability to bind or activate β receptors in cells expressing only this receptor, efficient β receptor activation by this ligand was readily observed in cells coexpressing α platelet-derived growth factor receptors (αPDGFRs). β receptor activation induced by PDGF-AB was shown to be dependent upon in vivo physical association of this receptor with αPDGFRs. Moreover, cross-linking analysis established the existence of PDGF-AB-induced βPDGFR dimers in vivo. All of these findings argue that initial PDGF-AB interaction with the αPDGFR induces conformational changes in the ligand or receptor that facilitates efficient recruitment of βPDGFR by this PDGF isoform.

Original languageEnglish
Pages (from-to)20232-20237
Number of pages6
JournalJournal of Biological Chemistry
Volume266
Issue number30
StatePublished - 1991
Externally publishedYes

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