Robust T-cell stimulation by Epstein-Barr virus-transformed B cells after antigen targeting to DEC-205

Carol S. Leung, Michael A. Maurer, Sonja Meixlsperger, Anne Lippmann, Cheolho Cheong, Jianmin Zuo, Tracey A. Haigh, Graham S. Taylor, Christian Münz

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

DEC-205 is a type I transmembrane multilectin receptor that is predominantly expressed on dendritic cells (DCs). Therefore, previous studies primarily focused on processing of DEC-205-targeted antigens by this potent antigen presenting cell type. Here we show that Epstein-Barr virus (EBV) transformed lymphoblastoid B-cell lines (LCLs) not only express DEC-205 at similar levels to DCs, but also efficiently present targeted EBV nuclear antigen 1 (EBNA1) and EBV-latent membrane protein 1 (LMP1) to EBNA1- and LMP1-specific CD4+and CD8+T-cell clones in vitro. Targeting of antigens to DEC-205 on B cells led to more efficient MHC class II than I loading, and stimulated T cells more efficiently than targeting to DEC-205 on DCs. Although LCLs internalized DEC-205- targeted antigens less efficiently than DCs, they retained them for longer time periods and delivered them to endosomal compartments that receive also B-cell receptor targeted proteins. This could facilitate prolonged T-cell stimulation and efficient MHC class II loading, and, indeed, CD4+T-cell expansion by DEC-205-targeted vaccination was significantly compromised in B-cell deficient mice. These studies suggest that B cells, activated by virus transformation or other means, can contribute to T-cell stimulation after DEC-205 targeting of antigens during vaccination.

Original languageEnglish
Pages (from-to)1584-1594
Number of pages11
JournalBlood
Volume121
Issue number9
DOIs
StatePublished - 28 Feb 2013
Externally publishedYes

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