RNAi Codex: a portal/database for short-hairpin RNA (shRNA) gene-silencing constructs.

A. Olson, N. Sheth, J. S. Lee, G. Hannon, R. Sachidanandam

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

Use of RNA interference (RNAi) in forward genetic screens is proliferating. Currently, short-interfering RNAs (siRNAs) and short-hairpin RNAs (shRNAs) are being used to silence genes to tease out functional information. It is becoming easier to harness RNAi to silence specific genes, owing to the development of libraries of readymade shRNA and siRNA gene-silencing constructs by using a variety of sources. RNAi Codex, which consists of a database of shRNA related information and an associated website, has been developed as a portal for publicly available shRNA resources and is accessible at http://codex.cshl.org. RNAi Codex currently holds data from the Hannon-Elledge shRNA library and allows the use of biologist-friendly gene names to access information on shRNA constructs that can silence the gene of interest. It is designed to hold user-contributed annotations and publications for each construct, as and when such data become available. We will describe features of RNAi Codex and explain the use of the tool.

Original languageEnglish
Pages (from-to)D153-157
JournalNucleic Acids Research
Volume34
Issue numberDatabase issue
DOIs
StatePublished - 1 Jan 2006
Externally publishedYes

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