RNA Pol II Assembly Affects ncRNA Expression

Ana I. Garrido-Godino, Ishaan Gupta, Vicent Pelechano, Francisco Navarro

Research output: Contribution to journalArticlepeer-review


RNA pol II assembly occurs in the cytoplasm before translocation of the enzyme to the nucleus. Affecting this assembly influences mRNA transcription in the nucleus and mRNA decay in the cytoplasm. However, very little is known about the consequences on ncRNA synthesis. In this work, we show that impairment of RNA pol II assembly leads to a decrease in cryptic non-coding RNAs (preferentially CUTs and SUTs). This alteration is partially restored upon overcoming the assembly defect. Notably, this drop in ncRNAs is only partially dependent on the nuclear exosome, which suggests a major specific effect of enzyme assembly. Our data also point out a defect in transcription termination, which leads us to propose that CTD phosphatase Rtr1 could be involved in this process.

Original languageEnglish
Article number507
JournalInternational Journal of Molecular Sciences
Issue number1
StatePublished - Jan 2024
Externally publishedYes


  • CUTs
  • NNS termination
  • RNA polymerases
  • RNA polymerases assembly
  • Rtr1 CTD phosphatase
  • SUTs
  • Saccharomyces cerevisiae
  • exosome
  • ncRNAs
  • transcription


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