RNA-interference-based functional genomics in mammalian cells: Reverse genetics coming of age

Jose Silva, Kenneth Chang, Gregory J. Hannon, Fabiola V. Rivas

Research output: Contribution to journalReview articlepeer-review

102 Scopus citations

Abstract

Sequencing of complete genomes has provided researchers with a wealth of information to study genome organization, genetic instability, and polymorphisms, as well as a knowledge of all potentially expressed genes. The identification of all genes encoded in the human genome opens the door for large-scale systematic gene silencing using small interfering RNAs (siRNAs) and short hairpin RNAs (shRNAs). With the recent development of siRNA and shRNA expression libraries, the application of RNAi technology to assign function to cancer genes and to delineate molecular pathways in which these genes affect in normal and transformed cells, will contribute significantly to the knowledge necessary to develop new and also improve existing cancer therapy.

Original languageEnglish
Pages (from-to)8401-8409
Number of pages9
JournalOncogene
Volume23
Issue number51 REV. ISS. 8
DOIs
StatePublished - 1 Nov 2004
Externally publishedYes

Keywords

  • Apoptosis
  • Cancer
  • Functional genomics
  • High throughput screening
  • RNAi
  • Synthetic lethality

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