RIG-I mediates an antiviral response to Crimean-Congo hemorrhagic fever virus

Jessica R. Spengler, Jenish R. Patel, Ayan K. Chakrabarti, Marko Zivcec, Adolfo García-Sastre, Christina F. Spiropoulou, Éric Bergeron

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

In the cytoplasm, the retinoic acid-inducible gene I (RIG-I) senses the RNA genomes of several RNA viruses. RIG-I binds to viral RNA, eliciting an antiviral response via the cellular adaptor MAVS. Crimean-Congo hemorrhagic fever virus (CCHFV), a negative- sense RNA virus with a 5'-monophosphorylated genome, is a highly pathogenic zoonotic agent with significant public health implications. We found that, during CCHFV infection, RIG-I mediated a type I interferon (IFN) response via MAVS. Interfering with RIG-I signaling reduced IFN production and IFN-stimulated gene expression and increased viral replication. Immunostimulatory RNA was isolated from CCHFV-infected cells and from virion preparations, and RIG-I coimmunoprecipitation of infected cell lysates isolated immunostimulatory CCHFV RNA. This report serves as the first description of a pattern recognition receptor for CCHFV and highlights a critical signaling pathway in the antiviral response to CCHFV.

Original languageEnglish
Pages (from-to)10219-10229
Number of pages11
JournalJournal of Virology
Volume89
Issue number20
DOIs
StatePublished - 2015

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