RIG-E, a human homolog of the murine Ly-6 family, is induced by retinoic acid during the differentiation of acute promyelocytic leukemia cell

Mao Mao, Man Yu, Jian Hua Tong, Jing Ye, Jun Zhu, Qiu Hua Huang, Gang Fu, Long Yu, Shou Yuan Zhao, Samuel Waxman, Michel Lanotte, Zhen Yi Wang, Jia Zhen Tan, Sai Juan Chan, Zhu Chen

Research output: Contribution to journalArticlepeer-review

96 Scopus citations

Abstract

In vivo all-trans-retinoic acid (ATRA), a differentiation inducer, is capable of causing clinical remission in about 90% of patients with acute promyelocytic leukemia (APL). The molecular basis for the differentiation of APL cells after treatment with ATRA remains obscure and may involve genes other than the known retinoid nuclear transcription factors. We report here the ATRA-induced gene expression in a cell line (NB4) derived from a patient with APL. By differential display-PCR, we isolated and characterized a novel gene (RIG-E) whose expression is up-regulated by ATRA. The gene is 4.0 kb long, consisting of four exons and three introns, and is localized on human chromosome region 8q24. The deduced amino acid sequence predicts a cell surface protein containing 20 amino acids at the N-terminal end corresponding to a signal peptide and an extracellular sequence containing 111 amino acids. The RIG-E coded protein shares some homology with CD59 and with a number of growth factor receptors. It shares high sequence homology with the murine LY- 6 multigene family, whose members are small cysteine-rich proteins differentially expressed in several hematopoietic cell lines and appear to function in signal transduction. It seems that so far RIG-E is the closest human homolog of the LY-6 family. Expression of RIG-E is not restricted to myeloid differentiation, because it is also present in thymocytes and in a number of other tissues at different levels.

Original languageEnglish
Pages (from-to)5910-5914
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume93
Issue number12
DOIs
StatePublished - 11 Jun 1996

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