Rift Valley fever virus infection induces activation of the NLRP3 inflammasome

Megan E. Ermler, Zachary Traylor, Krupen Patel, Stefan A. Schattgen, Sivapriya K. Vanaja, Katherine A. Fitzgerald, Amy G. Hise

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Inflammasome activation is gaining recognition as an important mechanism for protection during viral infection. Here, we investigate whether Rift Valley fever virus, a negative-strand RNA virus, can induce inflammasome responses and IL-1β processing in immune cells. We have determined that RVFV induces NLRP3 inflammasome activation in murine dendritic cells, and that this process is dependent upon ASC and caspase-1. Furthermore, absence of the cellular RNA helicase adaptor protein MAVS/IPS-1 significantly reduces extracellular IL-1β during infection. Finally, direct imaging using confocal microscopy shows that the MAVS protein co-localizes with NLRP3 in the cytoplasm of RVFV infected cells.

Original languageEnglish
Pages (from-to)174-180
Number of pages7
JournalVirology
Volume449
DOIs
StatePublished - 20 Jan 2014
Externally publishedYes

Keywords

  • ASC
  • Caspase-1
  • Dendritic cells
  • IL-1β
  • Inflammasome
  • Murine
  • NLRP3
  • Rift Valley fever virus
  • Virus

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