Reversed-phase ion-pair high-performance liquid chromatography of adriamycin and adriamycinol in rat serum and tissues

J. M. Gallo, C. T. Hung, D. G. Perrier

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

An ion-pair reversed-phase high-performance liquid chromatographic method is presented for the determination of adriamycin and adriamycinol concentrations in rat serum and tissues. The scheme for developing the chromatographic conditions is discussed. A good separation of adriamycinol and adriamycin was obtained by using a high sodium lauryl sulphate concentration in the mobile phase. The retention times of adriamycinol, adriamycin and daunomycin (internal standard) were less than 3 min with no interfering peaks from endogenous constituents. A protein precipitation method was used to prepare the serum and tissue samples for injection.

Original languageEnglish
Pages (from-to)483-490
Number of pages8
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume4
Issue number4
DOIs
StatePublished - 1986
Externally publishedYes

Keywords

  • Ion-pair HPLC
  • adriamycin and adriamycinol.

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