TY - JOUR
T1 - Retroviruses as probes for mammalian development
T2 - Allocation of cells to the somatic and germ cell lineages
AU - Soriano, Philippe
AU - Jaenisch, Rudolf
N1 - Funding Information:
We thank Doris Grotkopp for excellent technical assistance and all of our colleagues for critical comments on the manuscript. This work was supported by grant No. HD-19105 from the National Institutes of Health and by grant No. POl-CA38497 from the National Cancer Institute.
PY - 1986/7/4
Y1 - 1986/7/4
N2 - Preimplantation mouse embryos were infected with a recombinant retrovirus, which serves as a genetic marker for the progeny of an infected blastomere. Quantitative analysis of proviruses carried in mosaic animals indicated that the molarity of individual proviral bands was equal in almost all tissues. The cells that give rise to the embryo proper must therefore intermingle extensively before final tissue allocation to ensure equal contribution of founder cells to all somatic tissues. The distribution of molarities of individual proviruses suggested that somatic lineages are derived from at most eight founder cells. About half of the proviruses were present in the germ line and the somatic tissues of mosaic animals, while the remaining proviruses were found either in the germ line or in the somatic tissues, but not in both. Our results suggest that at least three cells form the germ line and are set aside prior to somatic tissue allocation.
AB - Preimplantation mouse embryos were infected with a recombinant retrovirus, which serves as a genetic marker for the progeny of an infected blastomere. Quantitative analysis of proviruses carried in mosaic animals indicated that the molarity of individual proviral bands was equal in almost all tissues. The cells that give rise to the embryo proper must therefore intermingle extensively before final tissue allocation to ensure equal contribution of founder cells to all somatic tissues. The distribution of molarities of individual proviruses suggested that somatic lineages are derived from at most eight founder cells. About half of the proviruses were present in the germ line and the somatic tissues of mosaic animals, while the remaining proviruses were found either in the germ line or in the somatic tissues, but not in both. Our results suggest that at least three cells form the germ line and are set aside prior to somatic tissue allocation.
UR - http://www.scopus.com/inward/record.url?scp=0022549490&partnerID=8YFLogxK
U2 - 10.1016/0092-8674(86)90856-1
DO - 10.1016/0092-8674(86)90856-1
M3 - Article
C2 - 3013418
AN - SCOPUS:0022549490
SN - 0092-8674
VL - 46
SP - 19
EP - 29
JO - Cell
JF - Cell
IS - 1
ER -