Retroviruses as probes for mammalian development: Allocation of cells to the somatic and germ cell lineages

Philippe Soriano, Rudolf Jaenisch

Research output: Contribution to journalArticlepeer-review

148 Scopus citations

Abstract

Preimplantation mouse embryos were infected with a recombinant retrovirus, which serves as a genetic marker for the progeny of an infected blastomere. Quantitative analysis of proviruses carried in mosaic animals indicated that the molarity of individual proviral bands was equal in almost all tissues. The cells that give rise to the embryo proper must therefore intermingle extensively before final tissue allocation to ensure equal contribution of founder cells to all somatic tissues. The distribution of molarities of individual proviruses suggested that somatic lineages are derived from at most eight founder cells. About half of the proviruses were present in the germ line and the somatic tissues of mosaic animals, while the remaining proviruses were found either in the germ line or in the somatic tissues, but not in both. Our results suggest that at least three cells form the germ line and are set aside prior to somatic tissue allocation.

Original languageEnglish
Pages (from-to)19-29
Number of pages11
JournalCell
Volume46
Issue number1
DOIs
StatePublished - 4 Jul 1986
Externally publishedYes

Fingerprint

Dive into the research topics of 'Retroviruses as probes for mammalian development: Allocation of cells to the somatic and germ cell lineages'. Together they form a unique fingerprint.

Cite this