Resveratrol selectively remodels soluble oligomers and fibrils of amyloid Aβ into off-pathway conformers

Ali Reza A. Ladiwala, Jason C. Lin, Shyam Sundhar Bale, Anna Marie Marcelino-Cruz, Moumita Bhattacharya, Jonathan S. Dordick, Peter M. Tessier

Research output: Contribution to journalArticlepeer-review

262 Scopus citations

Abstract

Misfolded proteins associated with diverse aggregation disorders assemble not only into a single toxic conformer but rather into a suite of aggregated conformers with unique biochemical properties and toxicities. To what extent small molecules can target and neutralize specific aggregated conformers is poorly understood. Therefore, we have investigated the capacity of resveratrol to recognize and remodel five conformers (monomers, soluble oligomers, non-toxic oligomers, fibrillar intermediates, and amyloid fibrils) of the Aβ1-42 peptide associated with Alzheimer disease. We find that resveratrol selectively remodels three of these conformers (soluble oligomers, fibrillar intermediates, and amyloid fibrils) into an alternative aggregated species that is non-toxic, high molecular weight, and unstructured. Surprisingly, resveratrol does not remodel non-toxic oligomers or accelerate Aβ monomer aggregation despite that both conformers possess random coil secondary structures indistinguishable from soluble oligomers and significantly different from their β-sheet rich, fibrillar counterparts. We expect that resveratrol and other small molecules with similar conformational specificity will aid in illuminating the conformational epitopes responsible for Aβ-mediated toxicity.

Original languageEnglish
Pages (from-to)24228-24237
Number of pages10
JournalJournal of Biological Chemistry
Volume285
Issue number31
DOIs
StatePublished - 30 Jul 2010
Externally publishedYes

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