Restorative effects of human neural stem cell grafts on the primate spinal cord

Ephron S. Rosenzweig; John H. Brock; Paul Lu; Hiromi Kumamaru; Ernesto A. Salegio; Ken Kadoya; Janet L. Weber; Justine J. Liang; Rod Moseanko; Stephanie Hawbecker; J. Russell Huie; Leif A. Havton; Yvette S. Nout-Lomas; Adam R. Ferguson; Michael S. Beattie; Jacqueline C. Bresnahan; Mark H. Tuszynski

doi:10.1038/nm.4502

Restorative effects of human neural stem cell grafts on the primate spinal cord

Ephron S. Rosenzweig, John H. Brock, Paul Lu, Hiromi Kumamaru, Ernesto A. Salegio, Ken Kadoya, Janet L. Weber, Justine J. Liang, Rod Moseanko, Stephanie Hawbecker, J. Russell Huie, Leif A. Havton, Yvette S. Nout-Lomas, Adam R. Ferguson, Michael S. Beattie, Jacqueline C. Bresnahan, Mark H. Tuszynski

Research output: Contribution to journal › Article › peer-review

236 Scopus citations

Abstract

We grafted human spinal cord-derived neural progenitor cells (NPCs) into sites of cervical spinal cord injury in rhesus monkeys (Macaca mulatta). Under three-drug immunosuppression, grafts survived at least 9 months postinjury and expressed both neuronal and glial markers. Monkey axons regenerated into grafts and formed synapses. Hundreds of thousands of human axons extended out from grafts through monkey white matter and synapsed in distal gray matter. Grafts gradually matured over 9 months and improved forelimb function beginning several months after grafting. These findings in a 'preclinical trial' support translation of NPC graft therapy to humans with the objective of reconstituting both a neuronal and glial milieu in the site of spinal cord injury.

Original language	English
Pages (from-to)	484-490
Number of pages	7
Journal	Nature Medicine
Volume	24
Issue number	4
DOIs	https://doi.org/10.1038/nm.4502
State	Published - 1 May 2018
Externally published	Yes

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Rosenzweig, E. S., Brock, J. H., Lu, P., Kumamaru, H., Salegio, E. A., Kadoya, K., Weber, J. L., Liang, J. J., Moseanko, R., Hawbecker, S., Huie, J. R., Havton, L. A., Nout-Lomas, Y. S., Ferguson, A. R., Beattie, M. S., Bresnahan, J. C., & Tuszynski, M. H. (2018). Restorative effects of human neural stem cell grafts on the primate spinal cord. Nature Medicine, 24(4), 484-490. https://doi.org/10.1038/nm.4502

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title = "Restorative effects of human neural stem cell grafts on the primate spinal cord",

abstract = "We grafted human spinal cord-derived neural progenitor cells (NPCs) into sites of cervical spinal cord injury in rhesus monkeys (Macaca mulatta). Under three-drug immunosuppression, grafts survived at least 9 months postinjury and expressed both neuronal and glial markers. Monkey axons regenerated into grafts and formed synapses. Hundreds of thousands of human axons extended out from grafts through monkey white matter and synapsed in distal gray matter. Grafts gradually matured over 9 months and improved forelimb function beginning several months after grafting. These findings in a 'preclinical trial' support translation of NPC graft therapy to humans with the objective of reconstituting both a neuronal and glial milieu in the site of spinal cord injury.",

author = "Rosenzweig, {Ephron S.} and Brock, {John H.} and Paul Lu and Hiromi Kumamaru and Salegio, {Ernesto A.} and Ken Kadoya and Weber, {Janet L.} and Liang, {Justine J.} and Rod Moseanko and Stephanie Hawbecker and Huie, {J. Russell} and Havton, {Leif A.} and Nout-Lomas, {Yvette S.} and Ferguson, {Adam R.} and Beattie, {Michael S.} and Bresnahan, {Jacqueline C.} and Tuszynski, {Mark H.}",

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Rosenzweig, ES, Brock, JH, Lu, P, Kumamaru, H, Salegio, EA, Kadoya, K, Weber, JL, Liang, JJ, Moseanko, R, Hawbecker, S, Huie, JR, Havton, LA, Nout-Lomas, YS, Ferguson, AR, Beattie, MS, Bresnahan, JC & Tuszynski, MH 2018, 'Restorative effects of human neural stem cell grafts on the primate spinal cord', Nature Medicine, vol. 24, no. 4, pp. 484-490. https://doi.org/10.1038/nm.4502

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AU - Lu, Paul

AU - Kumamaru, Hiromi

AU - Salegio, Ernesto A.

AU - Kadoya, Ken

AU - Weber, Janet L.

AU - Liang, Justine J.

AU - Moseanko, Rod

AU - Hawbecker, Stephanie

AU - Huie, J. Russell

AU - Havton, Leif A.

AU - Nout-Lomas, Yvette S.

AU - Ferguson, Adam R.

AU - Beattie, Michael S.

AU - Bresnahan, Jacqueline C.

AU - Tuszynski, Mark H.

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Restorative effects of human neural stem cell grafts on the primate spinal cord

Abstract

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