Response surface optimization of the heparosan N-deacetylation in producing bioengineered heparin

Zhenyu Wang, Jennifer Li, Samantha Cheong, Ujjwal Bhaskar, Onishi Akihiro, Fuming Zhang, Jonathan S. Dordick, Robert J. Linhardt

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

The chemical step in the chemoenzymatic synthesis of bioengineered heparin has been examined and optimized statistically using a response surface methodology. A four factor, two level full factorial design experiment and a three factor Box-Behnken design were carried out. The goal was to establish a method to prepare N-sulfo, N-acetyl heparosan of the desired N-acetyl content, number average molecular weight, and in maximum yield by controlling the reactant concentrations, reaction time and reaction temperature. The response surface models obtained were used to predict the reaction conditions required to optimally prepare N-sulfo, N-acetyl heparosan from Escherichia coli generated heparosan starting material of different molecular weights.

Original languageEnglish
Pages (from-to)188-196
Number of pages9
JournalJournal of Biotechnology
Volume156
Issue number3
DOIs
StatePublished - 10 Dec 2011
Externally publishedYes

Keywords

  • Bioengineered
  • Factorial design
  • Heparin
  • Heparosan
  • N-deacetylation

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