TY - JOUR
T1 - Respiratory tissue-associated commensal bacteria offer therapeutic potential against pneumococcal colonization
AU - Yildiz, Soner
AU - Bonifacio Lopes, João P.Pereira
AU - Bergé, Matthieu
AU - González-Ruiz, Victor
AU - Baud, Damian
AU - Kloehn, Joachim
AU - Boal-Carvalho, Inês
AU - Schaeren, Olivier P.
AU - Schotsaert, Michael
AU - Hathaway, Lucy J.
AU - Rudaz, Serge
AU - Viollier, Patrick H.
AU - Hapfelmeier, Siegfried
AU - Francois, Patrice
AU - Schmolke, Mirco
N1 - Publisher Copyright:
© Yildiz et al.
PY - 2020/12
Y1 - 2020/12
N2 - Under eubiotic conditions commensal microbes are known to provide a competitive barrier against invading bacterial pathogens in the intestinal tract, on the skin or on the vaginal mucosa. Here, we evaluate the role of lung microbiota in Pneumococcus colonization of the lungs. In eubiosis, the lungs of mice were dominantly colonized by Lactobacillus murinus. Differential analysis of 16S rRNA gene sequencing or L. murinus-specific qPCR of DNA from total organ homogenates vs.broncho alveolar lavages implicated tight association of these bacteria with the host tissue. Pure L. murinus conditioned culture medium inhibited growth and reduced the extension of pneumococcal chains. Growth inhibition in vitro was likely dependent on L. murinus-produced lactic acid, since pH neutralization of the conditioned medium aborted the antibacterial effect. Finally, we demonstrate that L. murinus provides a barrier against pneumococcal colonization in a respiratory dysbiosis model after an influenza A virus infection, when added therapeutically.
AB - Under eubiotic conditions commensal microbes are known to provide a competitive barrier against invading bacterial pathogens in the intestinal tract, on the skin or on the vaginal mucosa. Here, we evaluate the role of lung microbiota in Pneumococcus colonization of the lungs. In eubiosis, the lungs of mice were dominantly colonized by Lactobacillus murinus. Differential analysis of 16S rRNA gene sequencing or L. murinus-specific qPCR of DNA from total organ homogenates vs.broncho alveolar lavages implicated tight association of these bacteria with the host tissue. Pure L. murinus conditioned culture medium inhibited growth and reduced the extension of pneumococcal chains. Growth inhibition in vitro was likely dependent on L. murinus-produced lactic acid, since pH neutralization of the conditioned medium aborted the antibacterial effect. Finally, we demonstrate that L. murinus provides a barrier against pneumococcal colonization in a respiratory dysbiosis model after an influenza A virus infection, when added therapeutically.
UR - http://www.scopus.com/inward/record.url?scp=85097483609&partnerID=8YFLogxK
U2 - 10.7554/ELIFE.53581
DO - 10.7554/ELIFE.53581
M3 - Article
C2 - 33287959
AN - SCOPUS:85097483609
SN - 2050-084X
VL - 9
SP - 1
EP - 25
JO - eLife
JF - eLife
M1 - e53581
ER -