Resistance/response molecular signature for oral tongue squamous cell carcinoma

Amritha Suresh; Muhil Vannan; Dhanya Kumaran; Zeynep H. Gümüs; Priya Sivadas; Elango Erode Murugaian; Vikram Kekatpure; Subramanian Iyer; Kumarasamy Thangaraj; Moni Abraham Kuriakose

doi:10.3233/DMA-2012-0860

Resistance/response molecular signature for oral tongue squamous cell carcinoma

Amritha Suresh, Muhil Vannan, Dhanya Kumaran, Zeynep H. Gümüs, Priya Sivadas, Elango Erode Murugaian, Vikram Kekatpure, Subramanian Iyer, Kumarasamy Thangaraj, Moni Abraham Kuriakose

Research output: Contribution to journal › Review article › peer-review

28 Scopus citations

Abstract

Worldwide, the incidence of oral tongue cancer is on the rise, adding to the existing burden due to prevailing low survival and high recurrence rates. This study uses high-throughput expression profiling to identify candidate markers of resistance/response in patients with oral tongue cancer. Analysis of primary and post-treatment samples (12 tumor and 8 normal) by the Affymetrix platform (HG U133 plus 2) identified 119 genes as differentially regulated in recurrent tumors. The study groups had distinct profiles, with induction of immune response and apoptotic pathways in the non-recurrent and metastatic/invasiveness pathways in the recurrent group. Validation was carried out in tissues by Quantitative Real-Time PCR (QPCR) (n=30) and immunohistochemistry (IHC) (n=35) and in saliva by QPCR (n=37). The markers, COL5A1, HBB, IGLA and TSC individually and COL5A1 and HBB in combination had the best predictive power for treatment response in the patients. A subset of markers identified (COL5A1, ABCG1, MMP1, IL8, FN1) could be detected in the saliva of patients with oral cancers with their combined sensitivity and specificity being 0.65 and 0.87 respectively. The study thus emphasizes the extreme prognostic value of exploring markers of treatment resistance that are expressed in both tissue and saliva.

Original language	English
Pages (from-to)	51-64
Number of pages	14
Journal	Disease Markers
Volume	32
Issue number	1
DOIs	https://doi.org/10.3233/DMA-2012-0860
State	Published - 2012
Externally published	Yes

Keywords

Tongue cancer
biomarkers
gene expression
micro array
resistance
response
saliva

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10.3233/DMA-2012-0860

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title = "Resistance/response molecular signature for oral tongue squamous cell carcinoma",

abstract = "Worldwide, the incidence of oral tongue cancer is on the rise, adding to the existing burden due to prevailing low survival and high recurrence rates. This study uses high-throughput expression profiling to identify candidate markers of resistance/response in patients with oral tongue cancer. Analysis of primary and post-treatment samples (12 tumor and 8 normal) by the Affymetrix platform (HG U133 plus 2) identified 119 genes as differentially regulated in recurrent tumors. The study groups had distinct profiles, with induction of immune response and apoptotic pathways in the non-recurrent and metastatic/invasiveness pathways in the recurrent group. Validation was carried out in tissues by Quantitative Real-Time PCR (QPCR) (n=30) and immunohistochemistry (IHC) (n=35) and in saliva by QPCR (n=37). The markers, COL5A1, HBB, IGLA and TSC individually and COL5A1 and HBB in combination had the best predictive power for treatment response in the patients. A subset of markers identified (COL5A1, ABCG1, MMP1, IL8, FN1) could be detected in the saliva of patients with oral cancers with their combined sensitivity and specificity being 0.65 and 0.87 respectively. The study thus emphasizes the extreme prognostic value of exploring markers of treatment resistance that are expressed in both tissue and saliva.",

keywords = "Tongue cancer, biomarkers, gene expression, micro array, resistance, response, saliva",

author = "Amritha Suresh and Muhil Vannan and Dhanya Kumaran and G{\"u}m{\"u}s, {Zeynep H.} and Priya Sivadas and Murugaian, {Elango Erode} and Vikram Kekatpure and Subramanian Iyer and Kumarasamy Thangaraj and Kuriakose, {Moni Abraham}",

year = "2012",

doi = "10.3233/DMA-2012-0860",

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TY - JOUR

T1 - Resistance/response molecular signature for oral tongue squamous cell carcinoma

AU - Suresh, Amritha

AU - Vannan, Muhil

AU - Kumaran, Dhanya

AU - Gümüs, Zeynep H.

AU - Sivadas, Priya

AU - Murugaian, Elango Erode

AU - Kekatpure, Vikram

AU - Iyer, Subramanian

AU - Thangaraj, Kumarasamy

AU - Kuriakose, Moni Abraham

PY - 2012

Y1 - 2012

N2 - Worldwide, the incidence of oral tongue cancer is on the rise, adding to the existing burden due to prevailing low survival and high recurrence rates. This study uses high-throughput expression profiling to identify candidate markers of resistance/response in patients with oral tongue cancer. Analysis of primary and post-treatment samples (12 tumor and 8 normal) by the Affymetrix platform (HG U133 plus 2) identified 119 genes as differentially regulated in recurrent tumors. The study groups had distinct profiles, with induction of immune response and apoptotic pathways in the non-recurrent and metastatic/invasiveness pathways in the recurrent group. Validation was carried out in tissues by Quantitative Real-Time PCR (QPCR) (n=30) and immunohistochemistry (IHC) (n=35) and in saliva by QPCR (n=37). The markers, COL5A1, HBB, IGLA and TSC individually and COL5A1 and HBB in combination had the best predictive power for treatment response in the patients. A subset of markers identified (COL5A1, ABCG1, MMP1, IL8, FN1) could be detected in the saliva of patients with oral cancers with their combined sensitivity and specificity being 0.65 and 0.87 respectively. The study thus emphasizes the extreme prognostic value of exploring markers of treatment resistance that are expressed in both tissue and saliva.

AB - Worldwide, the incidence of oral tongue cancer is on the rise, adding to the existing burden due to prevailing low survival and high recurrence rates. This study uses high-throughput expression profiling to identify candidate markers of resistance/response in patients with oral tongue cancer. Analysis of primary and post-treatment samples (12 tumor and 8 normal) by the Affymetrix platform (HG U133 plus 2) identified 119 genes as differentially regulated in recurrent tumors. The study groups had distinct profiles, with induction of immune response and apoptotic pathways in the non-recurrent and metastatic/invasiveness pathways in the recurrent group. Validation was carried out in tissues by Quantitative Real-Time PCR (QPCR) (n=30) and immunohistochemistry (IHC) (n=35) and in saliva by QPCR (n=37). The markers, COL5A1, HBB, IGLA and TSC individually and COL5A1 and HBB in combination had the best predictive power for treatment response in the patients. A subset of markers identified (COL5A1, ABCG1, MMP1, IL8, FN1) could be detected in the saliva of patients with oral cancers with their combined sensitivity and specificity being 0.65 and 0.87 respectively. The study thus emphasizes the extreme prognostic value of exploring markers of treatment resistance that are expressed in both tissue and saliva.

KW - Tongue cancer

KW - biomarkers

KW - gene expression

KW - micro array

KW - resistance

KW - response

KW - saliva

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VL - 32

SP - 51

EP - 64

JO - Disease Markers

JF - Disease Markers

IS - 1

ER -

Resistance/response molecular signature for oral tongue squamous cell carcinoma

Abstract

Keywords

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