Resistance of different forms of cytochrome P-450 of rat liver to factors destabilizing the microsomal membrane

O. A. Gromova; N. E. Zakharova; V. V. Lyakhovich; I. B. Tsyrlov

doi:10.1007/BF00800740

Resistance of different forms of cytochrome P-450 of rat liver to factors destabilizing the microsomal membrane

O. A. Gromova, N. E. Zakharova, V. V. Lyakhovich, I. B. Tsyrlov

Icahn School of Medicine at Mount Sinai

Research output: Contribution to journal › Article › peer-review

Abstract

The effect of factors destabilizing the membrane of the liver microsomes on the spectral properties of cytochrome P-450 (P-448) was investigated in intact rats and rats receiving phenobarbital (PB) or 3-methylcholanthrene (MC). Considerable resistance of microsomes induced by PB and MC to enzymic and nonenzymic peroxidation of polyunsaturated fatty acids of membrane phospholipids was discovered. A clear difference was shown in the sensitivity of cytochrome P-448 and cytochrome P-450 of intact rats and rats receiving PB to in vitro treatment with sodium deoxycholate. The results indicate structural changes in the microsomal membrane during induction by PB and MC, which are two different types of inducers of the monooxygenases of the liver.

Original language	English
Pages (from-to)	653-656
Number of pages	4
Journal	Bulletin of Experimental Biology and Medicine
Volume	83
Issue number	5
DOIs	https://doi.org/10.1007/BF00800740
State	Published - May 1977

Keywords

cytochrome P-450
deoxycholate
induction
peroxidation of lipids

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10.1007/BF00800740

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title = "Resistance of different forms of cytochrome P-450 of rat liver to factors destabilizing the microsomal membrane",

abstract = "The effect of factors destabilizing the membrane of the liver microsomes on the spectral properties of cytochrome P-450 (P-448) was investigated in intact rats and rats receiving phenobarbital (PB) or 3-methylcholanthrene (MC). Considerable resistance of microsomes induced by PB and MC to enzymic and nonenzymic peroxidation of polyunsaturated fatty acids of membrane phospholipids was discovered. A clear difference was shown in the sensitivity of cytochrome P-448 and cytochrome P-450 of intact rats and rats receiving PB to in vitro treatment with sodium deoxycholate. The results indicate structural changes in the microsomal membrane during induction by PB and MC, which are two different types of inducers of the monooxygenases of the liver.",

keywords = "cytochrome P-450, deoxycholate, induction, peroxidation of lipids",

author = "Gromova, \{O. A.\} and Zakharova, \{N. E.\} and Lyakhovich, \{V. V.\} and Tsyrlov, \{I. B.\}",

year = "1977",

month = may,

doi = "10.1007/BF00800740",

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TY - JOUR

T1 - Resistance of different forms of cytochrome P-450 of rat liver to factors destabilizing the microsomal membrane

AU - Gromova, O. A.

AU - Zakharova, N. E.

AU - Lyakhovich, V. V.

AU - Tsyrlov, I. B.

PY - 1977/5

Y1 - 1977/5

N2 - The effect of factors destabilizing the membrane of the liver microsomes on the spectral properties of cytochrome P-450 (P-448) was investigated in intact rats and rats receiving phenobarbital (PB) or 3-methylcholanthrene (MC). Considerable resistance of microsomes induced by PB and MC to enzymic and nonenzymic peroxidation of polyunsaturated fatty acids of membrane phospholipids was discovered. A clear difference was shown in the sensitivity of cytochrome P-448 and cytochrome P-450 of intact rats and rats receiving PB to in vitro treatment with sodium deoxycholate. The results indicate structural changes in the microsomal membrane during induction by PB and MC, which are two different types of inducers of the monooxygenases of the liver.

AB - The effect of factors destabilizing the membrane of the liver microsomes on the spectral properties of cytochrome P-450 (P-448) was investigated in intact rats and rats receiving phenobarbital (PB) or 3-methylcholanthrene (MC). Considerable resistance of microsomes induced by PB and MC to enzymic and nonenzymic peroxidation of polyunsaturated fatty acids of membrane phospholipids was discovered. A clear difference was shown in the sensitivity of cytochrome P-448 and cytochrome P-450 of intact rats and rats receiving PB to in vitro treatment with sodium deoxycholate. The results indicate structural changes in the microsomal membrane during induction by PB and MC, which are two different types of inducers of the monooxygenases of the liver.

KW - cytochrome P-450

KW - deoxycholate

KW - induction

KW - peroxidation of lipids

UR - https://www.scopus.com/pages/publications/0017692789

U2 - 10.1007/BF00800740

DO - 10.1007/BF00800740

M3 - Article

AN - SCOPUS:0017692789

SN - 0007-4888

VL - 83

SP - 653

EP - 656

JO - Bulletin of Experimental Biology and Medicine

JF - Bulletin of Experimental Biology and Medicine

IS - 5

ER -

Resistance of different forms of cytochrome P-450 of rat liver to factors destabilizing the microsomal membrane

Abstract

Keywords

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