@inbook{aeecc2ded7514e0289ad1a722da17783,
title = "Rescue of Recombinant Newcastle Disease Virus Expressing Heterologous Genes",
abstract = "Reverse genetics allows for the generation of recombinant infectious viruses from viral sequences or complete viral genomes cloned into plasmids. Using reverse genetics, it is then possible to introduce changes in the genome of infectious viruses for multiple applications. Newcastle disease virus (NDV) is a non-segmented, negative-sense RNA virus that has been amenable to manipulation by reverse genetics for more than two decades. Since then, recombinant NDVs have been extensively used as viral vectors to express heterologous proteins. We describe the key steps required to design and introduce an additional transcription unit in the genome of the Newcastle disease virus for the efficient expression of a heterologous gene.",
keywords = "Codon optimization, Mononegavirales, Recombinant Newcastle disease virus, Reverse genetics, Virus-vectored vaccine",
author = "Arantza Cobela-Garc{\'i}a and Ignacio Mena and Adolfo Garc{\'i}a-Sastre",
note = "Publisher Copyright: {\textcopyright} The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024.",
year = "2024",
doi = "10.1007/978-1-0716-3533-9_3",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "37--46",
booktitle = "Methods in Molecular Biology",
}