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Replicative intermediate of hepatitis B virus in transfected murine fibroblasts

  • A. Z. Zelent
  • , M. A. Sells
  • , M. Shvartsman
  • , P. M. Price
  • , G. Acs

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

The NIH 3T3-derived cell line ψAM22b, which carries a defective Moloney murine leukemia virus, was transfected with a plasmid carrying the neo gene and two head-to-tail copies of the hepatitis B virus (HBV) genome positioned with opposing polarities. Both the two HBV dimers and the neo gene were located between two Moloney murine leukemia virus long terminal repeats. Poly(A)+ RNAs isolated from one clone that grew in the presence of G418 contained the two major classes of HBV-specific transcripts (3.5-kilobase pregenome and 2.1-kilobase mRNAs) in approximately equivalent amounts, which was reminiscent of the profiles of viral mRNAs from the livers of infected humans and chimpanzees.

Original languageEnglish
Pages (from-to)2921-2923
Number of pages3
JournalJournal of Virology
Volume61
Issue number9
DOIs
StatePublished - 1987

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