Renaturation of bacteriophage φX174 DNA-RNA hybrid: RNA length effect and nucleation rate constant

James R. Hutton; James G. Wetmur

doi:10.1016/0022-2836(73)90218-0

Renaturation of bacteriophage φX174 DNA-RNA hybrid: RNA length effect and nucleation rate constant

James R. Hutton
, James G. Wetmur

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

A one to one RNA-DNA hybrid was synthesized using bacteriophage φX174 DNA as a template for the Escherichia coli RNA polymerase system. The renaturation rate for the hybrid was found to vary as the square root of the molecular weight of the RNA. The RNA molecular weights were determined using a standardized dimethylsulfoxide, cesium sulfate equilibrium density gradient. The optimum nucleation rate constant for the renaturation of the hybrid in 0.4 m-sodium chloride was found to be 1.3 × 10⁵, as compared with to 1.7 × 10⁵ for DNA renaturation.

Original language	English
Pages (from-to)	495-500
Number of pages	6
Journal	Journal of Molecular Biology
Volume	77
Issue number	4
DOIs	https://doi.org/10.1016/0022-2836(73)90218-0
State	Published - 15 Jul 1973
Externally published	Yes

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@article{c6e9484f0d5346a98740d8bfcdfbfafe,

title = "Renaturation of bacteriophage φX174 DNA-RNA hybrid: RNA length effect and nucleation rate constant",

abstract = "A one to one RNA-DNA hybrid was synthesized using bacteriophage φX174 DNA as a template for the Escherichia coli RNA polymerase system. The renaturation rate for the hybrid was found to vary as the square root of the molecular weight of the RNA. The RNA molecular weights were determined using a standardized dimethylsulfoxide, cesium sulfate equilibrium density gradient. The optimum nucleation rate constant for the renaturation of the hybrid in 0.4 m-sodium chloride was found to be 1.3 × 105, as compared with to 1.7 × 105 for DNA renaturation.",

author = "Hutton, \{James R.\} and Wetmur, \{James G.\}",

note = "Funding Information: This research was supported by United States Public Health grant no. GM17215. One of us (J. R. H.) is a predoctoral trainee on United States Public Health grant no. GMO0722.",

year = "1973",

month = jul,

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doi = "10.1016/0022-2836(73)90218-0",

language = "English",

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journal = "Journal of Molecular Biology",

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T1 - Renaturation of bacteriophage φX174 DNA-RNA hybrid

T2 - RNA length effect and nucleation rate constant

AU - Hutton, James R.

AU - Wetmur, James G.

N1 - Funding Information: This research was supported by United States Public Health grant no. GM17215. One of us (J. R. H.) is a predoctoral trainee on United States Public Health grant no. GMO0722.

PY - 1973/7/15

Y1 - 1973/7/15

N2 - A one to one RNA-DNA hybrid was synthesized using bacteriophage φX174 DNA as a template for the Escherichia coli RNA polymerase system. The renaturation rate for the hybrid was found to vary as the square root of the molecular weight of the RNA. The RNA molecular weights were determined using a standardized dimethylsulfoxide, cesium sulfate equilibrium density gradient. The optimum nucleation rate constant for the renaturation of the hybrid in 0.4 m-sodium chloride was found to be 1.3 × 105, as compared with to 1.7 × 105 for DNA renaturation.

AB - A one to one RNA-DNA hybrid was synthesized using bacteriophage φX174 DNA as a template for the Escherichia coli RNA polymerase system. The renaturation rate for the hybrid was found to vary as the square root of the molecular weight of the RNA. The RNA molecular weights were determined using a standardized dimethylsulfoxide, cesium sulfate equilibrium density gradient. The optimum nucleation rate constant for the renaturation of the hybrid in 0.4 m-sodium chloride was found to be 1.3 × 105, as compared with to 1.7 × 105 for DNA renaturation.

UR - https://www.scopus.com/pages/publications/0015888699

U2 - 10.1016/0022-2836(73)90218-0

DO - 10.1016/0022-2836(73)90218-0

M3 - Article

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VL - 77

SP - 495

EP - 500

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

IS - 4

ER -

Renaturation of bacteriophage φX174 DNA-RNA hybrid: RNA length effect and nucleation rate constant

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