Removal of the superficial zone of bovine articular cartilage does not increase its frictional coefficient

R. Krishnan, M. Caligaris, R. L. Mauck, C. T. Hung, K. D. Costa, Gerard A. Ateshian

Research output: Contribution to journalArticlepeer-review

54 Scopus citations

Abstract

Objective: To investigate the role of the superficial zone in regulating the frictional response of articular cartilage. This zone contains the superficial protein (SZP), a proteoglycan synthesized exclusively by superficial zone chondrocytes and implicated in reducing the friction coefficient of cartilage. Design: Unconfined compression creep tests with sliding of cartilage against glass in saline were carried out on fresh bovine cylindrical plugs (∅6 mm, n = 35) obtained from 16 bovine shoulder joints (ages 1-3 months). In the first two experiments, friction tests were carried out before and after removal of the superficial zone (∼100 μm), in a control and treatment group, using two different applied load magnitudes (4.4 N and 22.2 N). In the third experiment, friction tests were conducted on intact surfaces and the corresponding microtomed deep zone of the same specimen. Results: In all tests the friction coefficient exhibited a transient response, increasing from a minimum value (μmin) to a near-equilibrium final value (μeq). No statistical change (P > 0.5) was found in μmin before and after removal of the superficial zone in both experiments 1 and 2. However, μeq was observed to decrease significantly (P < 0.001) after removal of the surface zone. Results from the third experiment confirm that μeq is even lower at the deep zone. Surface roughness measurements with atomic force microscopy (AFM) revealed an increase in surface roughness after microtoming. Immunohistochemical staining confirmed the presence of SZP in intact specimens and its removal in microtomed specimens. Conclusions: The topmost (∼100 μm) superficial zone of articular cartilage does not have special properties which enhances its frictional response.

Original languageEnglish
Pages (from-to)947-955
Number of pages9
JournalOsteoarthritis and Cartilage
Volume12
Issue number12
DOIs
StatePublished - Dec 2004
Externally publishedYes

Keywords

  • Atomic force microscopy
  • Cartilage
  • Friction
  • Interstitial fluid pressurization
  • Superficial zone protein

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