Abstract
The adsorption abilities of nine weakly basic anion exchange resins for propionic acid (PA) in vitamin B12 (VB12) fermentation broth have been evaluated. The static adsorption capacity on resin ZGA330 was found to be 44.9 mg/g dry beads at 12.6 g/L of PA. Dynamic state exchange trials, including the adsorption of glucose, amino acids and PA in the fermentation broth, were also carried out; these indicated that the adsorption of glucose and amino acids-the main nutrient components of the fermentation broth-was minimal. The dynamic adsorption capacity was 205.5 mg/g dry beads. By removal of propionic acid with ZGA330, the concentration of VB12 increased from 9.1 mg/L to 13.1 mg/L, an increase of 44%. This demonstrates that coupling of the fermentation process with separation and removal of propionic acid affords a significant improvement in the cell concentration and productivity of VB12.
Original language | English |
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Pages (from-to) | 70-74 |
Number of pages | 5 |
Journal | Beijing Huagong Daxue Xuebao (Ziran Kexueban)/Journal of Beijing University of Chemical Technology (Natural Science Edition) |
Volume | 35 |
Issue number | 3 |
State | Published - May 2008 |
Externally published | Yes |
Keywords
- Adsorption
- Anion exchange resin
- Propionic acid (PA)
- Vitamin B