Reliability of human cortical organoid generation

Se Jin Yoon, Lubayna S. Elahi, Anca M. Pașca, Rebecca M. Marton, Aaron Gordon, Omer Revah, Yuki Miura, Elisabeth M. Walczak, Gwendolyn M. Holdgate, H. Christina Fan, John R. Huguenard, Daniel H. Geschwind, Sergiu P. Pașca

Research output: Contribution to journalArticlepeer-review

203 Scopus citations


The differentiation of pluripotent stem cells in three-dimensional cultures can recapitulate key aspects of brain development, but protocols are prone to variable results. Here we differentiated multiple human pluripotent stem cell lines for over 100 d using our previously developed approach to generate brain-region-specific organoids called cortical spheroids and, using several assays, found that spheroid generation was highly reliable and consistent. We anticipate the use of this approach for large-scale differentiation experiments and disease modeling.

Original languageEnglish
Pages (from-to)75-78
Number of pages4
JournalNature Methods
Issue number1
StatePublished - 1 Jan 2019
Externally publishedYes


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