Release of nitric oxide by nerve stimulation in the human urogenital tract

Anna M. Leone; N. Peter Wiklund; Tomas Hökfelt; Lou Brundin; Salvador Moncada

doi:10.1097/00001756-199402000-00019

Release of nitric oxide by nerve stimulation in the human urogenital tract

Anna M. Leone, N. Peter Wiklund, Tomas Hökfelt, Lou Brundin, Salvador Moncada

Research output: Contribution to journal › Article › peer-review

55 Scopus citations

Abstract

Nerve-induced release of the nitric oxide (NO) breakdown products nitrite (NO₂-) and nitrate (NO₃-) and the histochemical localization of NO synthase in the human penile corpus cavemosum and urethra were studied. Relaxations induced by nerve stimulation were inhibited by N^ω-nitro-L-arginine methyl ester (L-NAME), an effect which was reversed by L-arginine. Relaxations elicited either by nerve stimulation or exogenous NO were accompanied by the appearance of equivalent amounts of NO₂- and NO₃- over a very similar time course. Nerve-induced release of NO₂- and NO₃- was inhibited by L-NAME. Histochemical studies showed NADPH diaphorase and NO-positive nerve fibres surrounding the arteries and smooth muscle bundles in the corpus cavernosum and the urethra. The results suggest that NO is a mediator for non-adrenergic non-cholinergic relaxation in the human urogenital tract.

Original language	English
Pages (from-to)	733-736
Number of pages	4
Journal	NeuroReport
Volume	5
Issue number	6
DOIs	https://doi.org/10.1097/00001756-199402000-00019
State	Published - Feb 1994
Externally published	Yes

Keywords

NADPH diaphorase
Nitric oxide
Nitric oxide synthase
Non-adrenergic non-cholinergic
Penile corpus cavemosum
Urethra

Access to Document

10.1097/00001756-199402000-00019

Cite this

@article{19c9163bc35040f4838a11063f9b61ed,

title = "Release of nitric oxide by nerve stimulation in the human urogenital tract",

abstract = "Nerve-induced release of the nitric oxide (NO) breakdown products nitrite (NO2-) and nitrate (NO3-) and the histochemical localization of NO synthase in the human penile corpus cavemosum and urethra were studied. Relaxations induced by nerve stimulation were inhibited by Nω-nitro-L-arginine methyl ester (L-NAME), an effect which was reversed by L-arginine. Relaxations elicited either by nerve stimulation or exogenous NO were accompanied by the appearance of equivalent amounts of NO2- and NO3- over a very similar time course. Nerve-induced release of NO2- and NO3- was inhibited by L-NAME. Histochemical studies showed NADPH diaphorase and NO-positive nerve fibres surrounding the arteries and smooth muscle bundles in the corpus cavernosum and the urethra. The results suggest that NO is a mediator for non-adrenergic non-cholinergic relaxation in the human urogenital tract.",

keywords = "NADPH diaphorase, Nitric oxide, Nitric oxide synthase, Non-adrenergic non-cholinergic, Penile corpus cavemosum, Urethra",

author = "Leone, {Anna M.} and Wiklund, {N. Peter} and Tomas H{\"o}kfelt and Lou Brundin and Salvador Moncada",

year = "1994",

month = feb,

doi = "10.1097/00001756-199402000-00019",

language = "English",

volume = "5",

pages = "733--736",

journal = "NeuroReport",

issn = "0959-4965",

publisher = "Lippincott Williams and Wilkins",

number = "6",

}

TY - JOUR

T1 - Release of nitric oxide by nerve stimulation in the human urogenital tract

AU - Leone, Anna M.

AU - Wiklund, N. Peter

AU - Hökfelt, Tomas

AU - Brundin, Lou

AU - Moncada, Salvador

PY - 1994/2

Y1 - 1994/2

N2 - Nerve-induced release of the nitric oxide (NO) breakdown products nitrite (NO2-) and nitrate (NO3-) and the histochemical localization of NO synthase in the human penile corpus cavemosum and urethra were studied. Relaxations induced by nerve stimulation were inhibited by Nω-nitro-L-arginine methyl ester (L-NAME), an effect which was reversed by L-arginine. Relaxations elicited either by nerve stimulation or exogenous NO were accompanied by the appearance of equivalent amounts of NO2- and NO3- over a very similar time course. Nerve-induced release of NO2- and NO3- was inhibited by L-NAME. Histochemical studies showed NADPH diaphorase and NO-positive nerve fibres surrounding the arteries and smooth muscle bundles in the corpus cavernosum and the urethra. The results suggest that NO is a mediator for non-adrenergic non-cholinergic relaxation in the human urogenital tract.

AB - Nerve-induced release of the nitric oxide (NO) breakdown products nitrite (NO2-) and nitrate (NO3-) and the histochemical localization of NO synthase in the human penile corpus cavemosum and urethra were studied. Relaxations induced by nerve stimulation were inhibited by Nω-nitro-L-arginine methyl ester (L-NAME), an effect which was reversed by L-arginine. Relaxations elicited either by nerve stimulation or exogenous NO were accompanied by the appearance of equivalent amounts of NO2- and NO3- over a very similar time course. Nerve-induced release of NO2- and NO3- was inhibited by L-NAME. Histochemical studies showed NADPH diaphorase and NO-positive nerve fibres surrounding the arteries and smooth muscle bundles in the corpus cavernosum and the urethra. The results suggest that NO is a mediator for non-adrenergic non-cholinergic relaxation in the human urogenital tract.

KW - NADPH diaphorase

KW - Nitric oxide

KW - Nitric oxide synthase

KW - Non-adrenergic non-cholinergic

KW - Penile corpus cavemosum

KW - Urethra

UR - http://www.scopus.com/inward/record.url?scp=0028324830&partnerID=8YFLogxK

U2 - 10.1097/00001756-199402000-00019

DO - 10.1097/00001756-199402000-00019

M3 - Article

C2 - 7515292

AN - SCOPUS:0028324830

SN - 0959-4965

VL - 5

SP - 733

EP - 736

JO - NeuroReport

JF - NeuroReport

IS - 6

ER -

Release of nitric oxide by nerve stimulation in the human urogenital tract

Abstract

Keywords

Access to Document

Fingerprint

Cite this