Regulation of Protein Kinase C Activity in Neuronal Differentiation Induced by the N-ras Oncogene in PC-12 Cells

J. C. Lacal, A. Cuadrado, J. E. Jones, R. Trotta, D. E. Burstein, T. Thomson, A. Pellicer

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

Expression of the N-ras oncogene under the control of the glucocorticoid-responsive promoter in the pheochromocytoma cell line UR61, a subline of PC-12 cells, has been used to investigate the differentiation process to neuronal cells triggered by ras oncogenes (I. Guerrero, A. Pellicer, and D. E. Burstein, Biochem. Biophys. Res. Commun. 150:1185-1192, 1988). Using ras-inducible cell lines, we observed that expression of the oncogenic N-ras p21 protein interferes with the ability of phorbol esters to induce downregulation of protein kinase C. This effect was associated with the appearance of immunologically detectable protein kinase C as well as the activity of the enzyme as analyzed either by binding of [3H]phorbol-12,13-dibutyrate in intact cells or by in vitro kinase activity. These results indicate a relationship between ras p21 and protein kinase C in neuronal differentiation in this model system. Comparison to the murine fibroblast system suggests that this relationship may be functional.

Original languageEnglish
Pages (from-to)2983-2990
Number of pages8
JournalMolecular and Cellular Biology
Volume10
Issue number6
DOIs
StatePublished - Jun 1990
Externally publishedYes

Fingerprint

Dive into the research topics of 'Regulation of Protein Kinase C Activity in Neuronal Differentiation Induced by the N-ras Oncogene in PC-12 Cells'. Together they form a unique fingerprint.

Cite this