Regulation of phototransduction responsiveness and retinal degeneration by a phospholipase D-generated signaling lipid

Mary M. LaLonde, Hilde Janssens, Erica Rosenbaum, Seok Yong Choi, J. Peter Gergen, Nansi J. Colley, William S. Stark, Michael A. Frohman

Research output: Contribution to journalArticlepeer-review

47 Scopus citations

Abstract

Drosophila melanogaster phototransduction proceeds via a phospholipase C (PLC)-triggered cascade of phosphatidylinositol (PI) lipid modifications, many steps of which remain undefined. We describe the involvement of the lipid phosphatidic acid and the enzyme that generates it, phospholipase D (Pld), in this process. Pldnull flies exhibit decreased light sensitivity as well as a heightened susceptibility to retinal degeneration. Pld overexpression rescues flies lacking PLC from light-induced, metarhodopsin-mediated degeneration and restores visual signaling in flies lacking the Pl transfer protein, which is a key player in the replenishment of the Pl 4,5-bisphosphate (PlP2) substrate used by PLC to transduce light stimuli into neurological signals. Altogether, these findings suggest that Pld facilitates phototransduction by maintaining adequate levels of PlP2 and by protecting the visual system from metarhodopsin-induced, low light degeneration.

Original languageEnglish
Pages (from-to)471-479
Number of pages9
JournalJournal of Cell Biology
Volume169
Issue number3
DOIs
StatePublished - 9 May 2005
Externally publishedYes

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