Regulation of ILT3 gene expression by processing of precursor transcripts in human endothelial cells

S. Kim-Schulze; T. Seki; G. Vlad; L. Scotto; J. Fan; P. C. Colombo; J. Liu; R. Cortesini; N. Suciu-Foca

doi:10.1111/j.1600-6143.2005.01162.x

Regulation of ILT3 gene expression by processing of precursor transcripts in human endothelial cells

S. Kim-Schulze, T. Seki, G. Vlad, L. Scotto, J. Fan, P. C. Colombo, J. Liu, R. Cortesini, N. Suciu-Foca

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34 Scopus citations

Abstract

Immunoglobulin-like transcript (ILT)-3 is a transmembrane receptor, which belongs to the immunoglobulin superfamily. In previous studies, we showed that allospecific CD8⁺CD28^- T suppressor cells (Ts) induce the expression of ILT3 in human endothelial cells (EC) rendering them tolerogenic. Using a polymerase chain reaction (PCR)-based approach, we now demonstrate by cell fractionation and sequencing studies that ILT3 precursor RNA is expressed and retained in nuclei of resting EC. Ts interaction with EC or exposure of EC to interleukin-10 (IL-10) and interferon alpha (IFN-α) triggers processing of ILT3 pre-mRNA. Western blot analysis showed that the expression of the mature ILT3 transcript is accompanied by production of ILT3 protein.

Original language	English
Pages (from-to)	76-82
Number of pages	7
Journal	American Journal of Transplantation
Volume	6
Issue number	1
DOIs	https://doi.org/10.1111/j.1600-6143.2005.01162.x
State	Published - Jan 2006
Externally published	Yes

Keywords

Activation-induced splicing
Immunoglobulin-like transcript 3
Tolerogenic endothelial cells

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10.1111/j.1600-6143.2005.01162.x

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title = "Regulation of ILT3 gene expression by processing of precursor transcripts in human endothelial cells",

abstract = "Immunoglobulin-like transcript (ILT)-3 is a transmembrane receptor, which belongs to the immunoglobulin superfamily. In previous studies, we showed that allospecific CD8+CD28- T suppressor cells (Ts) induce the expression of ILT3 in human endothelial cells (EC) rendering them tolerogenic. Using a polymerase chain reaction (PCR)-based approach, we now demonstrate by cell fractionation and sequencing studies that ILT3 precursor RNA is expressed and retained in nuclei of resting EC. Ts interaction with EC or exposure of EC to interleukin-10 (IL-10) and interferon alpha (IFN-α) triggers processing of ILT3 pre-mRNA. Western blot analysis showed that the expression of the mature ILT3 transcript is accompanied by production of ILT3 protein.",

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author = "S. Kim-Schulze and T. Seki and G. Vlad and L. Scotto and J. Fan and Colombo, {P. C.} and J. Liu and R. Cortesini and N. Suciu-Foca",

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AU - Kim-Schulze, S.

AU - Seki, T.

AU - Vlad, G.

AU - Scotto, L.

AU - Fan, J.

AU - Colombo, P. C.

AU - Liu, J.

AU - Cortesini, R.

AU - Suciu-Foca, N.

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AB - Immunoglobulin-like transcript (ILT)-3 is a transmembrane receptor, which belongs to the immunoglobulin superfamily. In previous studies, we showed that allospecific CD8+CD28- T suppressor cells (Ts) induce the expression of ILT3 in human endothelial cells (EC) rendering them tolerogenic. Using a polymerase chain reaction (PCR)-based approach, we now demonstrate by cell fractionation and sequencing studies that ILT3 precursor RNA is expressed and retained in nuclei of resting EC. Ts interaction with EC or exposure of EC to interleukin-10 (IL-10) and interferon alpha (IFN-α) triggers processing of ILT3 pre-mRNA. Western blot analysis showed that the expression of the mature ILT3 transcript is accompanied by production of ILT3 protein.

KW - Activation-induced splicing

KW - Immunoglobulin-like transcript 3

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Regulation of ILT3 gene expression by processing of precursor transcripts in human endothelial cells

Abstract

Keywords

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