TY - JOUR
T1 - Regional variation in the expression of bullous pemphigoid antigen and location of lesions in bullous pemphigoid
AU - Goldberg, D. J.
AU - Sabolinski, M.
AU - Bystryn, J. C.
N1 - Funding Information:
Studies were perfomed on two sets of normal human skin. Thirty· four specimens were obtained from the edges of surgically excised, benign skin lesions. Another 12 specimens were collected from multiple Manuscript received August 11, 1983; accepted for publication November 2, 1983. This work was supported by USP HS research grant AI 17365A-03. Reprint requests to: Jean-Claude Bystryn, M.D., Department of Dermatology, New York University School of Medicine, 560 First Avenue, New York, New York 10016. Abbreviations: BP: bullous pemphigoid PBS: phosphate-buffered saline 4-mm punch biopsies of normal skin in two cadavers taken within 48 h of death. No skin was taken from patients with blistering or autoimmune diseases. All specimens were immediately frozen in liquid nitrogen, cut in 4 I'll sections on a cryostat, and tested within 48 h.
PY - 1984
Y1 - 1984
N2 - The expression of bullous pemphigoid (BP) antigen in different locations on the body was estimated from the highest dilution of BP sera giving a positive reaction at a site by indirect immunofluorescence. Three sera with high titers (640-1280) of BP antibodies against monkey and guinea pig esophagus were reacted with 46 specimens of normal human skin. There was marked variation in the expression of BP antigen in skin obtained from different sites. The greatest expression of BP antigen was in skin obtained from the flexor surfaces of the arm, arm, leg, and thigh. All specimens from these sites reacted with high dilutions (1:640-1:2560) of BP antibodies. BP antigen was expressed least in skin specimens from scalp, face, and extensor surface of the arm. No BP antigen could be demonstrated in 17% of specimens from these locations and the remainder reacted only with low dilutions (1:40-1:160) of BP antibodies. Skin from trunk, knee, and extensor thigh had intermediate amounts of BP antigen. Similar results were seen with all 3 BP sera. Similar reactions were observed in multiple skin specimens obtained from different sites in the same individual. Of note is that the greatest expression of BP antigen was in areas commonly involved in this disease (flexural surfaces), whereas the least was seen in areas (scalp, face, extensor arm) which are rarely involved. The apparent correlation between the expression of BP antigen and the location of lesions in bullous pemphigoid, suggests that BP antigen expression may play a role in the distribution of skin lesions in this disease.
AB - The expression of bullous pemphigoid (BP) antigen in different locations on the body was estimated from the highest dilution of BP sera giving a positive reaction at a site by indirect immunofluorescence. Three sera with high titers (640-1280) of BP antibodies against monkey and guinea pig esophagus were reacted with 46 specimens of normal human skin. There was marked variation in the expression of BP antigen in skin obtained from different sites. The greatest expression of BP antigen was in skin obtained from the flexor surfaces of the arm, arm, leg, and thigh. All specimens from these sites reacted with high dilutions (1:640-1:2560) of BP antibodies. BP antigen was expressed least in skin specimens from scalp, face, and extensor surface of the arm. No BP antigen could be demonstrated in 17% of specimens from these locations and the remainder reacted only with low dilutions (1:40-1:160) of BP antibodies. Skin from trunk, knee, and extensor thigh had intermediate amounts of BP antigen. Similar results were seen with all 3 BP sera. Similar reactions were observed in multiple skin specimens obtained from different sites in the same individual. Of note is that the greatest expression of BP antigen was in areas commonly involved in this disease (flexural surfaces), whereas the least was seen in areas (scalp, face, extensor arm) which are rarely involved. The apparent correlation between the expression of BP antigen and the location of lesions in bullous pemphigoid, suggests that BP antigen expression may play a role in the distribution of skin lesions in this disease.
UR - https://www.scopus.com/pages/publications/0021258343
U2 - 10.1111/1523-1747.ep12260627
DO - 10.1111/1523-1747.ep12260627
M3 - Article
C2 - 6368699
AN - SCOPUS:0021258343
SN - 0022-202X
VL - 82
SP - 326
EP - 328
JO - Journal of Investigative Dermatology
JF - Journal of Investigative Dermatology
IS - 4
ER -